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Literature summary extracted from

  • Chen, L.; Yu, C.; Zhou, X.; Zhang, Y.
    Rational introduction of disulfide bond to enhance optimal temperature of Lipomyces starkeyi alpha-dextranase expressed in Pichia pastoris (2009), J. Microbiol. Biotechnol., 19, 1506-1513.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11 expressed in Pichia pastoris strain GS115 Lipomyces starkeyi

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.11 D279C/S289C the mutant shows a more than 13°C improvement on its optimal temperature compared to the wild type enzyme Lipomyces starkeyi
3.2.1.11 D279C/S289C the mutant shows a more than 3°C improvement on its optimal temperature compared to the wild type enzyme Lipomyces starkeyi
3.2.1.11 T245C/N248C the mutant shows a more than 3°C improvement on its optimal temperature compared to the wild type enzyme Lipomyces starkeyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.11 67200
-
 calculated from amino acid sequence Lipomyces starkeyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.11 dextran + H2O Lipomyces starkeyi
-
 oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Lipomyces starkeyi
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 13.66
-
 wild type enzyme, at pH 4.5, 30°C Lipomyces starkeyi
3.2.1.11 14.93
-
 mutant enzyme D279C/S289C, at pH 4.5, 43°C Lipomyces starkeyi
3.2.1.11 25.6
-
 mutant enzyme T245C/N248C, at pH 5.0, 33°C Lipomyces starkeyi
3.2.1.11 44.52
-
 mutant enzyme D279C/S289C, at pH 5.0, 33°C Lipomyces starkeyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Lipomyces starkeyi oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.11 alpha-dextranase
-
 Lipomyces starkeyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 30
-
-
 Lipomyces starkeyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 4.5
-
-
 Lipomyces starkeyi