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Literature summary extracted from

  • Cao, Y.; Wang, Y.; Luo, H.; Shi, P.; Meng, K.; Zhou, Z.; Zhang, Z.; Yao, B.
    Molecular cloning and expression of a novel protease-resistant GH-36 alpha-galactosidase from Rhizopus sp. F78 ACCC 30795 (2009), J. Microbiol. Biotechnol., 19, 1295-1300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 EDTA the activity of recombinant Aga-F78 is significantly enhanced in the presence of EDTA Rhizopus sp. ACCC 30795

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Escherichia coli BL21(DE3) cells Rhizopus sp. ACCC 30795

General Stability

EC Number General Stability Organism
3.2.1.22 the enzyme is protease-resistant, when combined with trypsin, the enzyme retained over 90% degradability to soybean meal Rhizopus sp. ACCC 30795

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ strong inhibitor Rhizopus sp. ACCC 30795
3.2.1.22 Hg2+ strong inhibitor Rhizopus sp. ACCC 30795
3.2.1.22 Mn2+ strong inhibitor Rhizopus sp. ACCC 30795
3.2.1.22 SDS strong inhibitor Rhizopus sp. ACCC 30795

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Cr3+ the activity of recombinant Aga-F78 is significantly enhanced in the presence of Cr3+ Rhizopus sp. ACCC 30795
3.2.1.22 Fe3+ the activity of recombinant Aga-F78 is significantly enhanced in the presence of Fe3+ Rhizopus sp. ACCC 30795
3.2.1.22 additional information the addition of other metal ions or chemicals has little or no effect on the activity Rhizopus sp. ACCC 30795
3.2.1.22 Pb2+ the activity of recombinant Aga-F78 is significantly enhanced in the presence of Pb2+ Rhizopus sp. ACCC 30795

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 82000
-
 3 * 82000, SDS-PAGE Rhizopus sp. ACCC 30795
3.2.1.22 210000
-
 gel filtration Rhizopus sp. ACCC 30795

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Rhizopus sp. ACCC 30795 C7SEV1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 Ni-NTA column chromatography Rhizopus sp. ACCC 30795

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 56.61
-
 after 2461.3fold purification, using 4-nitrophenyl alpha-D-galactoside as substrate, at 37°C Rhizopus sp. ACCC 30795

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactoside + H2O
-
 Rhizopus sp. ACCC 30795 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 melibiose + H2O
-
 Rhizopus sp. ACCC 30795 D-galactose + D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Rhizopus sp. ACCC 30795 sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Rhizopus sp. ACCC 30795 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 homotrimer 3 * 82000, SDS-PAGE Rhizopus sp. ACCC 30795

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Aga-F78
-
 Rhizopus sp. ACCC 30795

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
 Rhizopus sp. ACCC 30795

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50 60 the recombinant enzyme loses about 60% of the initial activity after incubation at 50°C for 30 min and is completely inactivated at 60°C for 5 min Rhizopus sp. ACCC 30795

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.8
-
-
 Rhizopus sp. ACCC 30795

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 5 10 highly pH stable over the pH range 5.0-10.0 Rhizopus sp. ACCC 30795

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Rhizopus sp. ACCC 30795 calculated from amino acid sequence
-
 5.4