EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.18 | NaCl | concentration of 0.5 - 1 M NaCl | Rhodothermus marinus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.18 | DMSO | about 22.9% decreased enzyme activity, 10% | Rhodothermus marinus | |
2.4.1.18 | EDTA | about 44.2% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | ethanol | about 57.2% decreased enzyme activity, 10% | Rhodothermus marinus | |
2.4.1.18 | methanol | about 45.2% decreased enzyme activity, 10% | Rhodothermus marinus | |
2.4.1.18 | NaCl | concetration of 2-4 M NaCl | Rhodothermus marinus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.18 | extracellular | - |
Rhodothermus marinus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.18 | Ba2+ | about 2.5% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Ca2+ | about 3.6% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Cu2+ | about 16.4% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Fe2+ | about 58.3% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Li+ | - |
Rhodothermus marinus | |
2.4.1.18 | Mg2+ | 15% increased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Mn2+ | about 53.5% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
2.4.1.18 | Zn2+ | about 61.8% decreased enzyme activity, 5 mM | Rhodothermus marinus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.18 | Rhodothermus marinus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.18 | ammonium sulfate, Q-Sepharose column, ultrafiltration, gel filtration with Superdex 200 column, native polyacrylamide gel elektrophoresis | Rhodothermus marinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.18 | amylose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
2.4.1.18 | Maltohexaose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
2.4.1.18 | maltopentaose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
2.4.1.18 | Pullulan | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.18 | RMEBE | - |
Rhodothermus marinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 80 | - |
- |
Rhodothermus marinus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 40 | 90 | - |
Rhodothermus marinus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 75 | - |
fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min | Rhodothermus marinus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 6 | - |
- |
Rhodothermus marinus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 3 | 9 | - |
Rhodothermus marinus |