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Literature summary extracted from

  • Mazzei, R.; Giorno, L.; Piacentini, E.; Mazzuca, S.; Drioli, E.
    Kinetic study of a biocatalytic membrane reactor containing immobilized beta-glucosidase for the hydrolysis of oleuropein (2009), J. Membr. Sci., 339, 215-223.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.38
-
 oleuropein enzyme immobilized on polysulphone capillary membranes, at pH 6.5 and 25°C Prunus dulcis
3.2.1.21 0.48
-
 oleuropein free enzyme, at pH 6.5 and 25°C Prunus dulcis
3.2.1.206 3.8
-
 oleuropein polysulfone capillary membrane-immobilized enzyme, at pH 6.5 and 25°C Prunus dulcis
3.2.1.206 4.8
-
 oleuropein free enzyme, at pH 6.5 and 25°C Prunus dulcis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.206 65000
-
 x * 65000, SDS-PAGE Prunus dulcis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.206 oleuropein + H2O Prunus dulcis
-
 oleuropein aglycone + beta-D-glucopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Prunus dulcis
-
-
-
3.2.1.206 Prunus dulcis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 seed
-
 Prunus dulcis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 oleuropein + H2O
-
 Prunus dulcis oleuropein aglycone + D-glucopyranose
-
 ?
3.2.1.21 salicin + H2O
-
 Prunus dulcis beta-D-glucose + 2-(hydroxymethyl)phenol
-
 ?
3.2.1.206 oleuropein + H2O
-
 Prunus dulcis oleuropein aglycone + beta-D-glucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.206 ? x * 65000, SDS-PAGE Prunus dulcis