BRENDA - Enzyme Database show

Cloning, characterization, and inhibition studies of a beta-carbonic anhydrase from Brucella suis

Joseph, P.; Turtaut, F.; Ouahrani-Bettache, S.; Montero, J.L.; Nishimori, I.; Minakuchi, T.; Vullo, D.; Scozzafava, A.; Koehler, S.; Winum, J.Y.; Supuran, C.T.; J. Med. Chem. 53, 2277-2285 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli BL21(DE3) cells
Brucella suis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Brucella suis
4.2.1.1
2-hydrazinylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4,5-dichloro-benzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3,5-dibromobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3,5-diiodobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Brucella suis
4.2.1.1
4-Aminobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-hydrazinylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4-methylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
-
Brucella suis
4.2.1.1
4-sulfamoylbenzoic acid
-
Brucella suis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Brucella suis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
acetazolamide
-
Brucella suis
4.2.1.1
benzolamide
-
Brucella suis
4.2.1.1
brinzolamide
-
Brucella suis
4.2.1.1
celecoxib
best CA 1 inhibitor
Brucella suis
4.2.1.1
dorzolamide
best CA 1 inhibitor
Brucella suis
4.2.1.1
ethoxzolamide
best CA 1 inhibitor
Brucella suis
4.2.1.1
indisulam
-
Brucella suis
4.2.1.1
methazolamide
-
Brucella suis
4.2.1.1
sulpiride
best CA 1 inhibitor
Brucella suis
4.2.1.1
topiramate
-
Brucella suis
4.2.1.1
valdecoxib
best CA 1 inhibitor
Brucella suis
4.2.1.1
zonisamide
-
Brucella suis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
25000
-
2 * 25000, SDS-PAGE
Brucella suis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Brucella suis
Q8FVP0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
Talon Co2+-affinity resin column chromatography, gel filtration
Brucella suis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705063
Brucella suis
H2CO3
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.1
homodimer
2 * 25000, SDS-PAGE
Brucella suis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
640000
-
CO2
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000017
-
ethoxzolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000018
-
celecoxib
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000019
-
sulpiride
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000019
-
valdecoxib
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000021
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000021
-
dorzolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000027
-
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000033
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000048
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000063
-
acetazolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00007
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000186
-
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000243
-
4-amino-3,5-dibromobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00034
-
4-sulfamoylbenzoic acid
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000345
-
4-amino-3,5-diiodobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000745
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000754
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000768
-
4-(aminomethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0008
-
4-amino-3-chlorobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000865
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00088
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00094
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.001035
-
2-hydrazinylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00105
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00107
-
4-amino-3-fluorobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00121
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00143
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00158
-
4-methylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0024
-
4-hydrazinylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0025
-
4-Aminobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00483
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00587
-
2-aminobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli BL21(DE3) cells
Brucella suis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Brucella suis
4.2.1.1
2-hydrazinylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4,5-dichloro-benzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3,5-dibromobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3,5-diiodobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Brucella suis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Brucella suis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Brucella suis
4.2.1.1
4-Aminobenzenesulfonamide
-
Brucella suis
4.2.1.1
4-hydrazinylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4-methylbenzenesulfonamide
-
Brucella suis
4.2.1.1
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
-
Brucella suis
4.2.1.1
4-sulfamoylbenzoic acid
-
Brucella suis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Brucella suis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Brucella suis
4.2.1.1
acetazolamide
-
Brucella suis
4.2.1.1
benzolamide
-
Brucella suis
4.2.1.1
brinzolamide
-
Brucella suis
4.2.1.1
celecoxib
best CA 1 inhibitor
Brucella suis
4.2.1.1
dorzolamide
best CA 1 inhibitor
Brucella suis
4.2.1.1
ethoxzolamide
best CA 1 inhibitor
Brucella suis
4.2.1.1
indisulam
-
Brucella suis
4.2.1.1
methazolamide
-
Brucella suis
4.2.1.1
sulpiride
best CA 1 inhibitor
Brucella suis
4.2.1.1
topiramate
-
Brucella suis
4.2.1.1
valdecoxib
best CA 1 inhibitor
Brucella suis
4.2.1.1
zonisamide
-
Brucella suis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000017
-
ethoxzolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000018
-
celecoxib
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000019
-
sulpiride
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000019
-
valdecoxib
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000021
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000021
-
dorzolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000027
-
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000033
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000048
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000063
-
acetazolamide
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00007
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000186
-
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000243
-
4-amino-3,5-dibromobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00034
-
4-sulfamoylbenzoic acid
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000345
-
4-amino-3,5-diiodobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000745
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000754
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000768
-
4-(aminomethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0008
-
4-amino-3-chlorobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.000865
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00088
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00094
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.001035
-
2-hydrazinylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00105
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00107
-
4-amino-3-fluorobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00121
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00143
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00158
-
4-methylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0024
-
4-hydrazinylbenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.0025
-
4-Aminobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00483
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
4.2.1.1
0.00587
-
2-aminobenzenesulfonamide
isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
25000
-
2 * 25000, SDS-PAGE
Brucella suis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
Talon Co2+-affinity resin column chromatography, gel filtration
Brucella suis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705063
Brucella suis
H2CO3
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.1
homodimer
2 * 25000, SDS-PAGE
Brucella suis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
640000
-
CO2
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
39000
-
CO2
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
39000
-
CO2
in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl
Brucella suis