Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Joseph, P.; Turtaut, F.; Ouahrani-Bettache, S.; Montero, J.L.; Nishimori, I.; Minakuchi, T.; Vullo, D.; Scozzafava, A.; Koehler, S.; Winum, J.Y.; Supuran, C.T.
    Cloning, characterization, and inhibition studies of a beta-carbonic anhydrase from Brucella suis (2010), J. Med. Chem., 53, 2277-2285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli BL21(DE3) cells Brucella suis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 2-aminobenzenesulfonamide
-
 Brucella suis
4.2.1.1 2-hydrazinylbenzenesulfonamide
-
 Brucella suis
4.2.1.1 4,5-dichloro-benzene-1,3-disulfonamide
-
 Brucella suis
4.2.1.1 4-(2-aminoethyl)benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-(2-hydroxyethyl)benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-(aminomethyl)benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-(hydroxymethyl)benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-3,5-dibromobenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-3,5-diiodobenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-3-chlorobenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-3-fluorobenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
 Brucella suis
4.2.1.1 4-amino-6-chlorobenzene-1,3-disulfonamide
-
 Brucella suis
4.2.1.1 4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
 Brucella suis
4.2.1.1 4-Aminobenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-hydrazinylbenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-methylbenzenesulfonamide
-
 Brucella suis
4.2.1.1 4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
-
 Brucella suis
4.2.1.1 4-sulfamoylbenzoic acid
-
 Brucella suis
4.2.1.1 4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
 Brucella suis
4.2.1.1 5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 5-amino-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
 Brucella suis
4.2.1.1 acetazolamide
-
 Brucella suis
4.2.1.1 benzolamide
-
 Brucella suis
4.2.1.1 brinzolamide
-
 Brucella suis
4.2.1.1 celecoxib best CA 1 inhibitor Brucella suis
4.2.1.1 dorzolamide best CA 1 inhibitor Brucella suis
4.2.1.1 ethoxzolamide best CA 1 inhibitor Brucella suis
4.2.1.1 indisulam
-
 Brucella suis
4.2.1.1 methazolamide
-
 Brucella suis
4.2.1.1 sulpiride best CA 1 inhibitor Brucella suis
4.2.1.1 topiramate
-
 Brucella suis
4.2.1.1 valdecoxib best CA 1 inhibitor Brucella suis
4.2.1.1 zonisamide
-
 Brucella suis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 25000
-
 2 * 25000, SDS-PAGE Brucella suis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Brucella suis A0A0H3GB68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Talon Co2+-affinity resin column chromatography, gel filtration Brucella suis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Brucella suis H2CO3
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 homodimer 2 * 25000, SDS-PAGE Brucella suis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-carbonic anhydrase
-
 Brucella suis
4.2.1.1 CA 1
-
 Brucella suis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 640000
-
 CO2 in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.000017
-
 ethoxzolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000018
-
 celecoxib in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000019
-
 valdecoxib in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000019
-
 sulpiride in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000021
-
 dorzolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000021
-
 4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000027
-
 4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000033
-
 5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000048
-
 6-hydroxy-1,3-benzothiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000063
-
 acetazolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00007
-
 5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000186
-
 5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000243
-
 4-amino-3,5-dibromobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00034
-
 4-sulfamoylbenzoic acid isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000345
-
 4-amino-3,5-diiodobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000745
-
 4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000754
-
 4-(hydroxymethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000768
-
 4-(aminomethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.0008
-
 4-amino-3-chlorobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.000865
-
 4-(2-hydroxyethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00088
-
 4-(2-aminoethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00094
-
 4-amino-6-chlorobenzene-1,3-disulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.001035
-
 2-hydrazinylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00105
-
 4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00107
-
 4-amino-3-fluorobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00121
-
 5-amino-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00143
-
 5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00158
-
 4-methylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.0024
-
 4-hydrazinylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.0025
-
 4-Aminobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00483
-
 4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
4.2.1.1 0.00587
-
 2-aminobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.1 39000
-
 CO2 in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis