BRENDA - Enzyme Database show

Carbonic anhydrase inhibitors. Cloning, characterization, and inhibition studies of a new beta-carbonic anhydrase from Mycobacterium tuberculosis

Nishimori, I.; Minakuchi, T.; Vullo, D.; Scozzafava, A.; Innocenti, A.; Supuran, C.T.; J. Med. Chem. 52, 3116-3120 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-amino-pyrimidin-4-yl-sulfanilamide
best inhibitor
Mycobacterium tuberculosis
4.2.1.1
2-aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
3-(4-sulfamoylphenyl)propanoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-Aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-methylbenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-sulfamoylbenzoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
acetazolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
benzolamide
-
Mycobacterium tuberculosis
4.2.1.1
brinzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
celecoxib
-
Mycobacterium tuberculosis
4.2.1.1
dichlorophenamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
dorzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
ethoxzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
indisulam
-
Mycobacterium tuberculosis
4.2.1.1
methazolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
saccharin
-
Mycobacterium tuberculosis
4.2.1.1
sulpiride
-
Mycobacterium tuberculosis
4.2.1.1
sulthiame
-
Mycobacterium tuberculosis
4.2.1.1
topiramate
-
Mycobacterium tuberculosis
4.2.1.1
valdecoxib
-
Mycobacterium tuberculosis
4.2.1.1
zonisamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Mycobacterium tuberculosis
-
-
-
4.2.1.1
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
-
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705034
Mycobacterium tuberculosis
H2CO3
-
-
-
r
4.2.1.1
CO2 + H2O
-
705034
Mycobacterium tuberculosis H37Rv
H2CO3
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
430000
-
CO2
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000091
-
2-amino-pyrimidin-4-yl-sulfanilamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000091
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000104
-
acetazolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000137
-
dorzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00017
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000186
-
4-amino-3-bromobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000201
-
brinzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000208
-
zonisamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000338
-
benzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000562
-
methazolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000594
-
ethoxzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000611
-
dichlorophenamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00075
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000853
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000905
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00151
-
4-amino-3-chlorobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00172
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00221
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00235
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00251
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00302
-
topiramate
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0031
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00342
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00492
-
4-methylbenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00551
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00581
-
4-amino-3-iodobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00624
-
2-aminobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00672
-
sulthiame
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00702
-
4-methylbenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00711
-
4-Aminobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00715
-
saccharin
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00732
-
4-amino-3-bromobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00733
-
4-(aminomethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0074
-
4-sulfamoylbenzoic acid
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00748
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00752
-
4-amino-3-chlorobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0076
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00763
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00771
-
4-amino-3-iodobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00776
-
celecoxib
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00781
-
valdecoxib
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00782
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00783
-
3-(4-sulfamoylphenyl)propanoic acid
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00784
-
indisulam
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0079
-
4-amino-3-fluorobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00792
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00792
-
sulpiride
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00793
-
3-(4-sulfamoylphenyl)propanoic acid
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0081
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00821
-
4-sulfamoylbenzoic acid
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00869
-
4-(aminomethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00874
-
4-amino-3-fluorobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00923
-
2-aminobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00984
-
4-Aminobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01154
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01265
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0217
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli
Mycobacterium tuberculosis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-amino-pyrimidin-4-yl-sulfanilamide
best inhibitor
Mycobacterium tuberculosis
4.2.1.1
2-aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
3-(4-sulfamoylphenyl)propanoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-Aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-methylbenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-sulfamoylbenzoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
acetazolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
benzolamide
-
Mycobacterium tuberculosis
4.2.1.1
brinzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
celecoxib
-
Mycobacterium tuberculosis
4.2.1.1
dichlorophenamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
dorzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
ethoxzolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
indisulam
-
Mycobacterium tuberculosis
4.2.1.1
methazolamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
4.2.1.1
saccharin
-
Mycobacterium tuberculosis
4.2.1.1
sulpiride
-
Mycobacterium tuberculosis
4.2.1.1
sulthiame
-
Mycobacterium tuberculosis
4.2.1.1
topiramate
-
Mycobacterium tuberculosis
4.2.1.1
valdecoxib
-
Mycobacterium tuberculosis
4.2.1.1
zonisamide
effective, submicromolar inhibition
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000091
-
2-amino-pyrimidin-4-yl-sulfanilamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000091
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000104
-
acetazolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000137
-
dorzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00017
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000186
-
4-amino-3-bromobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000201
-
brinzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000208
-
zonisamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000338
-
benzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000562
-
methazolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000594
-
ethoxzolamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000611
-
dichlorophenamide
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00075
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000853
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000905
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00151
-
4-amino-3-chlorobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00172
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00221
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00235
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00251
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00302
-
topiramate
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0031
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00342
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00492
-
4-methylbenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00551
-
4-(2-hydroxyethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00581
-
4-amino-3-iodobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00624
-
2-aminobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00672
-
sulthiame
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00702
-
4-methylbenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00711
-
4-Aminobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00715
-
saccharin
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00732
-
4-amino-3-bromobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00733
-
4-(aminomethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0074
-
4-sulfamoylbenzoic acid
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00748
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00752
-
4-amino-3-chlorobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0076
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00763
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00771
-
4-amino-3-iodobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00776
-
celecoxib
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00781
-
valdecoxib
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00782
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00783
-
3-(4-sulfamoylphenyl)propanoic acid
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00784
-
indisulam
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0079
-
4-amino-3-fluorobenzenesulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00792
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00792
-
sulpiride
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00793
-
3-(4-sulfamoylphenyl)propanoic acid
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0081
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00821
-
4-sulfamoylbenzoic acid
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00869
-
4-(aminomethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00874
-
4-amino-3-fluorobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00923
-
2-aminobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(2-aminoethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(hydroxymethyl)benzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00984
-
4-Aminobenzenesulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01154
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01265
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
isoform CA 1, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0217
-
4-amino-6-chlorobenzene-1,3-disulfonamide
isoform CA 3, at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
-
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705034
Mycobacterium tuberculosis
H2CO3
-
-
-
r
4.2.1.1
CO2 + H2O
-
705034
Mycobacterium tuberculosis H37Rv
H2CO3
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
430000
-
CO2
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
40000
-
CO2
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
40000
-
CO2
at 20°C, pH 8.3, in 20 mM Tris-HCl and 20 mM NaCl
Mycobacterium tuberculosis