BRENDA - Enzyme Database show

Molecular cloning, characterization, and inhibition studies of the Rv1284 beta-carbonic anhydrase from Mycobacterium tuberculosis with sulfonamides and a sulfamate

Minakuchi, T.; Nishimori, I.; Vullo, D.; Scozzafava, A.; Supuran, C.T.; J. Med. Chem. 52, 2226-2232 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
3-(4-sulfamoylphenyl)propanoic acid
-
Mycobacterium tuberculosis
4.2.1.1
3-bromosulfanilamide
-
Mycobacterium tuberculosis
4.2.1.1
4,5-dichloro-benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-Aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-methylbenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-sulfamoylbenzoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
acetazolamide
-
Mycobacterium tuberculosis
4.2.1.1
benzolamide
-
Mycobacterium tuberculosis
4.2.1.1
brinzolamide
-
Mycobacterium tuberculosis
4.2.1.1
celecoxib
-
Mycobacterium tuberculosis
4.2.1.1
dorzolamide
-
Mycobacterium tuberculosis
4.2.1.1
ethoxzolamide
-
Mycobacterium tuberculosis
4.2.1.1
indisulam
-
Mycobacterium tuberculosis
4.2.1.1
methazolamide
-
Mycobacterium tuberculosis
4.2.1.1
saccharin
-
Mycobacterium tuberculosis
4.2.1.1
sulpiride
-
Mycobacterium tuberculosis
4.2.1.1
sulthiame
-
Mycobacterium tuberculosis
4.2.1.1
topiramate
-
Mycobacterium tuberculosis
4.2.1.1
valdecoxib
-
Mycobacterium tuberculosis
4.2.1.1
zonisamide
-
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
glutathione Sepharose 4B column chromatography and sulfonamide affinity chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705027
Mycobacterium tuberculosis
H2CO3
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
390000
-
CO2
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000097
-
indisulam
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000186
-
4-amino-3-bromobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000481
-
acetazolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
topiramate
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000744
-
dorzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00075
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000781
-
methazolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00081
-
benzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000839
-
brinzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000853
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000872
-
4,5-dichloro-benzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000905
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00103
-
ethoxzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00172
-
4-amino-6-chlorobenzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0023
-
sulpiride
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00492
-
4-methylbenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00516
-
sulthiame
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00551
-
4-(2-hydroxyethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00748
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00752
-
4-amino-3-chlorobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00771
-
4-amino-3-iodobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00793
-
3-(4-sulfamoylphenyl)propanoic acid
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00796
-
saccharin
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0081
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00821
-
4-sulfamoylbenzoic acid
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00869
-
4-(aminomethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00874
-
4-amino-3-fluorobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00923
-
2-aminobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(2-aminoethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(hydroxymethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00984
-
4-Aminobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01035
-
celecoxib
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01154
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01265
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01297
-
valdecoxib
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.02868
-
zonisamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
3-(4-sulfamoylphenyl)propanoic acid
-
Mycobacterium tuberculosis
4.2.1.1
3-bromosulfanilamide
-
Mycobacterium tuberculosis
4.2.1.1
4,5-dichloro-benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-Aminobenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-methylbenzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
4-sulfamoylbenzoic acid
-
Mycobacterium tuberculosis
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mycobacterium tuberculosis
4.2.1.1
acetazolamide
-
Mycobacterium tuberculosis
4.2.1.1
benzolamide
-
Mycobacterium tuberculosis
4.2.1.1
brinzolamide
-
Mycobacterium tuberculosis
4.2.1.1
celecoxib
-
Mycobacterium tuberculosis
4.2.1.1
dorzolamide
-
Mycobacterium tuberculosis
4.2.1.1
ethoxzolamide
-
Mycobacterium tuberculosis
4.2.1.1
indisulam
-
Mycobacterium tuberculosis
4.2.1.1
methazolamide
-
Mycobacterium tuberculosis
4.2.1.1
saccharin
-
Mycobacterium tuberculosis
4.2.1.1
sulpiride
-
Mycobacterium tuberculosis
4.2.1.1
sulthiame
-
Mycobacterium tuberculosis
4.2.1.1
topiramate
-
Mycobacterium tuberculosis
4.2.1.1
valdecoxib
-
Mycobacterium tuberculosis
4.2.1.1
zonisamide
-
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000097
-
indisulam
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000186
-
4-amino-3-bromobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000481
-
acetazolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000612
-
topiramate
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000744
-
dorzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00075
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000781
-
methazolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00081
-
benzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000839
-
brinzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000853
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000872
-
4,5-dichloro-benzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.000905
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00103
-
ethoxzolamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00172
-
4-amino-6-chlorobenzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0023
-
sulpiride
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00492
-
4-methylbenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00516
-
sulthiame
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00551
-
4-(2-hydroxyethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00748
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00752
-
4-amino-3-chlorobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00771
-
4-amino-3-iodobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00793
-
3-(4-sulfamoylphenyl)propanoic acid
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00796
-
saccharin
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.0081
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00821
-
4-sulfamoylbenzoic acid
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00869
-
4-(aminomethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00874
-
4-amino-3-fluorobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00923
-
2-aminobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(2-aminoethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00956
-
4-(hydroxymethyl)benzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.00984
-
4-Aminobenzenesulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01035
-
celecoxib
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01154
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01265
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.01297
-
valdecoxib
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
4.2.1.1
0.02868
-
zonisamide
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
glutathione Sepharose 4B column chromatography and sulfonamide affinity chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
705027
Mycobacterium tuberculosis
H2CO3
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
390000
-
CO2
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
37000
-
CO2
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
37000
-
CO2
at 20°C and pH 8.3, in 20 mM Tris-HCl buffer and 20 mM NaCl
Mycobacterium tuberculosis