EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | expression of His-tagged mutant V77A/I130M/I138V in Escherichia colii strain M15 | Bacillus anthracis |
1.5.1.3 | recombinant expression of the enzyme | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.1.3 | purified recombinant mutant V77A/I130M/I138V enzyme in complex with inhibitor 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine, hanging-drop vapor diffusion in 25% w/v PEG 10,000, 0.1 M MES, pH 6.5, at an equal ratio of protein to crystallization solution. Microseeding is used to obtain isolated crystals in 10% w/v PEG 10000 and 0.1 M MES, pH 6.5, at a protein concentration of 10 mg/ml, improved by 15% ethylene glycol. X-ray diffraction structure determination and analysis at 2.5 A resolution | Bacillus anthracis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | V77A/I130M/I138V | site-directed mutagenesis | Bacillus anthracis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | 2,4-diamino-5-(3-phenylprop-1-ynyl)-6-ethylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-(3-phenylprop-1-ynyl)-6-ethylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(2,3-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(2,3-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-methylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-methylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-npropylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-npropylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]pyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]pyrimidine | - |
Homo sapiens | |
1.5.1.3 | 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Bacillus anthracis | |
1.5.1.3 | 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | - |
Homo sapiens | |
1.5.1.3 | methotrexate | - |
Bacillus anthracis | |
1.5.1.3 | methotrexate | - |
Homo sapiens | |
1.5.1.3 | additional information | inhibitor design based on a trimethoprim-like scaffold, overview | Bacillus anthracis | |
1.5.1.3 | additional information | inhibitor design based on a trimethoprim-like scaffold, overview | Homo sapiens | |
1.5.1.3 | trimethoprim | - |
Bacillus anthracis | |
1.5.1.3 | trimethoprim | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Homo sapiens | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Bacillus anthracis | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Bacillus anthracis | Q81R22 | - |
- |
1.5.1.3 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | recombinant enzyme | Homo sapiens |
1.5.1.3 | recombinant His-tagged mutant V77A/I130M/I138V from Escherichia colii strain M15 by nickel affinity chromatography | Bacillus anthracis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Bacillus anthracis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.3 | DHFR | - |
Homo sapiens |
1.5.1.3 | DHFR | - |
Bacillus anthracis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 25 | - |
assay at | Homo sapiens |
1.5.1.3 | 25 | - |
assay at | Bacillus anthracis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7 | - |
assay at | Homo sapiens |
1.5.1.3 | 7 | - |
assay at | Bacillus anthracis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Homo sapiens | |
1.5.1.3 | NADPH | - |
Bacillus anthracis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.000057 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.00013 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(2,3-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.00036 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-(3-phenylprop-1-ynyl)-6-ethylpyrimidine | |
1.5.1.3 | 0.00089 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.00094 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.00118 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-npropylpyrimidine | |
1.5.1.3 | 0.00128 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.0013 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-methylpyrimidine | |
1.5.1.3 | 0.0013 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-methylpyrimidine | |
1.5.1.3 | 0.0017 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]pyrimidine | |
1.5.1.3 | 0.0032 | - |
pH 7.0, 25°C | Homo sapiens | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]pyrimidine | |
1.5.1.3 | 0.0032 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-(3-phenylprop-1-ynyl)-6-ethylpyrimidine | |
1.5.1.3 | 0.0055 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(2,5-dimethoxyphenyl)prop-1-ynyl]-6-npropylpyrimidine | |
1.5.1.3 | 0.0092 | - |
pH 7.0, 25°C | Bacillus anthracis | 2,4-diamino-5-[3-(2,3-dimethoxyphenyl)prop-1-ynyl]-6-ethylpyrimidine | |
1.5.1.3 | 0.071 | - |
pH 7.0, 25°C | Bacillus anthracis | trimethoprim | |
1.5.1.3 | 0.12 | - |
pH 7.0, 25°C | Homo sapiens | trimethoprim |