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Literature summary extracted from

  • Hiramine, Y.; Emoto, H.; Takasuga, S.; Hiramatsu, R.
    Novel acyl-coenzyme A:monoacylglycerol acyltransferase (MGAT) plays an important role in hepatic triacylglycerol secretion (2010), J. Lipid Res., 51, 1424-1431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.22 PCR-amplification, transfection and expression of CHO cells with FLAG-tagged enzyme cDNA plasmid Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.22 additional information knockdown of hepatic lysophosphatidylglycerol acyltransferase 1 gene expression in transfected CHO cells by an adenovirus construct of short hairpin RNA (shRNA) Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.22 shRNA-adenovirus short hairpin RNA adenovirus construct to knockdown mouse lysophosphatidylglycerol acyltransferase 1, injected via tail vein Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.22 43000
-
sequence analysis, 370 amino acids Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.22 2-acyl-sn-glycerol + acetyl-CoA Mus musculus
-
diacylglycerol + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.22 Mus musculus
-
7 weeks old male diabetic (db/db) and control (db/m) mice, 7 days adjusted
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.22 heart
-
Mus musculus
-
2.3.1.22 intestine
-
Mus musculus
-
2.3.1.22 kidney
-
Mus musculus
-
2.3.1.22 liver most highly expressed, used for experiments Mus musculus
-
2.3.1.22 skin
-
Mus musculus
-
2.3.1.22 thymus
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.22 2-acyl-sn-glycerol + acetyl-CoA
-
Mus musculus diacylglycerol + CoA
-
?
2.3.1.22 2-acyl-sn-glycerol + palmitoyl-CoA in vitro assay in lysate of transfected CHO cells or of microsome preparation of mouse liver, 2-monoacylglycerol is preferred over 1-monoacylglycerol, 25°C, pH 6.4 Mus musculus diacylglycerol + CoA
-
?
2.3.1.22 additional information no significant production of phosphatidic acid (substrate lysophosphatidic acid), triacylglycerol (diacylglycerol), or cholesterol ester (substrate cholesterol), 25°C, pH 6.4 Mus musculus ?
-
?
2.3.1.22 rac-1-monoacylglycerol + palmitoyl-CoA in vitro assay in lysate of transfected CHO cells, 25°C, pH 6.4 Mus musculus diacylglycerol + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.22 acyl-coenzyme A:monoacylglycerol acyltransferase
-
Mus musculus
2.3.1.22 LPGAT1 two splice isoforms Mus musculus
2.3.1.22 lysophosphatidylglycerol acyltransferase 1
-
Mus musculus
2.3.1.22 MGAT
-
Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.22 additional information
-
infection with 60000000000 particles per animal (dose limit of no effect of control particles) leads to 83% decreased mRNA levels on day 5, 92% decrease on day 16 post-injection, associated with reduces hepatic MGAT and LPGAT activities (pH 6.4, 25°C), slight body weight reduction despite normal food consumption throughout most of the experimental treatment period (only 15% reduction on day 2), reduced serum triacylglycerols and cholesterol but increased total cholesterol accumulation in the liver Mus musculus shRNA-adenovirus

Expression

EC Number Organism Comment Expression
2.3.1.22 Mus musculus the infection with a short hairpin RNA-adenovirus construct lowers the lysophosphatidylglycerol acyltransferase 1 expression in diabetic (db/db) mice compared to a control virus infection in a dose-dependent manner, causing a marked reduction in serum triacylglycerol and cholesterol levels, and a significant increase in hepatic cholesterol levels down
2.3.1.22 Mus musculus hepatic LPGAT1 expression in diabetic (db/db) mice is 2fold higher than in control (db/m), consistent with 1.5fold higher hepatic monoacylglycerol acyltransferase activity up

General Information

EC Number General Information Comment Organism
2.3.1.22 metabolism significant role in hepatic triacylglycerol synthesis and secretion in diabetic mice (db/db) Mus musculus