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Literature summary extracted from

  • Yuki, K.; Shindou, H.; Hishikawa, D.; Shimizu, T.
    Characterization of mouse lysophosphatidic acid acyltransferase 3: an enzyme with dual functions in the testis (2009), J. Lipid Res., 50, 860-869.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 LPAAT3, phylogenetic analysis, expression of the FLAG-tagged enzyme in CHO-K1 cells Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 endoplasmic reticulum
-
 Mus musculus 5783
-
2.3.1.51 additional information subcellular distribution of LPAAT3, overview Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.51 37000
-
 x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE Mus musculus
2.3.1.51 43300
-
 x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Mus musculus
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Mus musculus C57/BL6J
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate Mus musculus
-
 CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate Mus musculus C57/BL6J
-
 CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information Mus musculus mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview ?
-
 ?
2.3.1.51 additional information Mus musculus C57/BL6J mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Mus musculus C4B4E7 C57BL/6J mice
-
2.3.1.51 Mus musculus C57/BL6J C4B4E7 C57BL/6J mice
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.51 adipose tissue low expression level Mus musculus
-
2.3.1.51 epididymis low expression level Mus musculus
-
2.3.1.51 heart low expression level Mus musculus
-
2.3.1.51 kidney low expression level Mus musculus
-
2.3.1.51 additional information tissue distribution of LPAAT3 expression, overview Mus musculus
-
2.3.1.51 testis high expression level, expression of the enzyme in the testis increases significantly in an age-dependent manner Mus musculus
-
2.3.1.51 TM-4 cell testicular cell line Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.51 additional information
-
 substrate specificity, overview Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors Mus musculus CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Mus musculus C57/BL6J CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors Mus musculus C57/BL6J CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Mus musculus C57/BL6J CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-octadecenyl-2-lysophosphatidylinositol
-
 Mus musculus CoA + 1-octadecenyl-2-arachidonoylphosphatidylinositol
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
-
 Mus musculus CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylinositol
-
 Mus musculus CoA + 1-palmitoyl-2-arachidonoylphosphatidylinositol
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidic acid
-
 Mus musculus CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information substrate specificity, overview Mus musculus ?
-
 ?
2.3.1.51 additional information mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview Mus musculus ?
-
 ?
2.3.1.51 additional information substrate specificity, overview Mus musculus C57/BL6J ?
-
 ?
2.3.1.51 additional information mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview Mus musculus C57/BL6J ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.51 ? x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 AGPAT3
-
 Mus musculus
2.3.1.51 LPAAT3
-
 Mus musculus
2.3.1.51 lysophosphatidic acid acyltransferase 3
-
 Mus musculus
2.3.1.51 More the enzyme belongs to the 1-acylglycerol-3-phosphate O-acyltransferase, AGPAT, family Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 7.4
-
 assay at Mus musculus

Expression

EC Number Organism Comment Expression
2.3.1.51 Mus musculus expression of the enzyme in the testis increases significantly in an age-dependent manner, which is regulated by induction through beta-estradiol, in TM4 cells up

General Information

EC Number General Information Comment Organism
2.3.1.51 metabolism the enzyme provides an an alternative important possibility to produce phosphatidylinositol in the testis Mus musculus