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Literature summary extracted from
- 5-Lipoxygenase: mechanisms of regulation (2009), J. Lipid Res., 50 Suppl, S40-S45.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.34 | 1,25(OH)2D3 | gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells | Homo sapiens |  |
| 1.13.11.34 | CLP | can upregulate and modulate the 5-lipoxygenase pathway in vitro | Homo sapiens | |
| 1.13.11.34 | additional information | in the mouse 5-lipoxygenase gene there are no GC-boxes in tandem, the core promoter contains only one Sp1/3 binding site | Mus musculus | |
| 1.13.11.34 | transforming growth factor beta | gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells | Homo sapiens |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.13.11.34 | drug development | gender difference in biosynthesis of leukotrienes (inflammatory mediators) may lead to new possibilities regarding development and use of 5-lipoxygenase inhibitors | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.34 | ABT-761 | - |
Homo sapiens | |
| 1.13.11.34 | cholesterol | addition to a membrane preperation in vitro reduces 5-lipoxygenase activity by half. Cholesterol sulfate can inhibit 5-lipoxygenase in intact cells | Homo sapiens | |
| 1.13.11.34 | mithramycin | reduces endogenous 5-lipoxygenase in Mono Mac 6 cell, which blocks GC-boxes in the proximal part of the 5-lipoxygenase gene promoter | Homo sapiens | |
| 1.13.11.34 | Zileutin | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.34 | cytosol | resides in the cytosol of resting neutrophils from females | Homo sapiens | 5829 | - |
| 1.13.11.34 | membrane | translocates from the cytosol to the membrane upon stimulation in female neutrophils | Homo sapiens | 16020 | - |
| 1.13.11.34 | nucleus | resides in a nuclear soluble compartment associated with chromatin | Rattus norvegicus | 5634 | - |
| 1.13.11.34 | nucleus | resides in a nuclear soluble compartment associated with chromatin. In male neutrophils, a substantial part of 5-lipoxygenase is located at the perinuclear region already in resting cells, only marginally redistributes upon stimulation | Homo sapiens | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.34 | Ca2+ | activates | Homo sapiens | |
| 1.13.11.34 | Fe2+ | - |
Homo sapiens | |
| 1.13.11.34 | Mg2+ | activates | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.34 | Homo sapiens | - |
- |
- |
| 1.13.11.34 | Mus musculus | - |
- |
- |
| 1.13.11.34 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.34 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.34 | B-lymphocyte | - |
Homo sapiens | - |
| 1.13.11.34 | basophilic leukemia cell | - |
Rattus norvegicus | - |
| 1.13.11.34 | dendritic cell | - |
Homo sapiens | - |
| 1.13.11.34 | eosinophil | - |
Homo sapiens | - |
| 1.13.11.34 | foam cell | atherosclerotic tissue | Homo sapiens | - |
| 1.13.11.34 | HL-60 cell | the 5-lipoxygenase core promoter is unmethylated, expression of 5-lipoxygenase protein upon differentiation | Homo sapiens | - |
| 1.13.11.34 | leukocyte | - |
Homo sapiens | - |
| 1.13.11.34 | macrophage | - |
Homo sapiens | - |
| 1.13.11.34 | mast cell | - |
Homo sapiens | - |
| 1.13.11.34 | Mono-Mac-6 cell | - |
Homo sapiens | - |
| 1.13.11.34 | monocyte | - |
Homo sapiens | - |
| 1.13.11.34 | neutrophil | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.34 | arachidonate + O2 | - |
Homo sapiens | (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.34 | 5-lipoxygenase | - |
Mus musculus |
| 1.13.11.34 | 5-lipoxygenase | - |
Homo sapiens |
| 1.13.11.34 | 5-lipoxygenase | - |
Rattus norvegicus |
| 1.13.11.34 | 5-LO | - |
Mus musculus |
| 1.13.11.34 | 5-LO | - |
Homo sapiens |
| 1.13.11.34 | 5-LO | - |
Rattus norvegicus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.13.11.34 | Homo sapiens | DNA methylation is responsible for suppression of 5-lipoxygenase expression in most cell types | down |
| 1.13.11.34 | Homo sapiens | aberrant methylation may lead to upregulated 5-lipoxygenase expression in tumor cells. The 5-lipoxygenase core promoter is completely methylated in cell lines U-937 and HL-60TB, which do not express 5-lipoxygenase. Treatment with the demethylating agent 5-aza-2'deoxycytidine restores 5-lipoxygenase expression | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.34 | physiological function | catalyzes two steps in biosynthesis of leukotrienes, a group of lipid mediators of inflammation derived from arachidonic acid, which are used as antagonists in treatment of asthma. A potential role also in atherosclerosis | Homo sapiens |
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