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Literature summary extracted from

  • Ogawa, S.; Yoshidomi, T.; Shirabe, T.; Yoshimura, E.
    HPLC method for the determination of phytochelatin synthase activity specific for soft metal ion chelators (2010), J. Inorg. Biochem., 104, 442-445.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.15 analysis a HPLC method for the analysis of the activity of phytochelatin synthase is developed Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 into the vector pET-28b for expression in Escherichia coli BL21 Rosetta DE3 pLysS cells Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.15 Cd2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.15 56500
-
 determined by SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Arabidopsis thaliana Q9S7Z3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.15 on a Ni2+ affinity column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.15 4.09
-
 10 ng/ml rAtPCS1 Arabidopsis thaliana
2.3.2.15 6.6
-
 30 ng/ml rAtPCS1 Arabidopsis thaliana
2.3.2.15 12.6
-
 50 ng/ml rAtPCS1 Arabidopsis thaliana
2.3.2.15 18.4
-
 10 ng/ml rAtPCS1, 100 ng/ml bovine serum albumin, BSA Arabidopsis thaliana
2.3.2.15 20.9
-
 1 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA Arabidopsis thaliana
2.3.2.15 24.9
-
 100 ng/ml rAtPCS1 Arabidopsis thaliana
2.3.2.15 25.9
-
 200 ng/ml rAtPCS1 Arabidopsis thaliana
2.3.2.15 30
-
 10 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 AtPCS1
-
 Arabidopsis thaliana
2.3.2.15 PCS1
-
 Arabidopsis thaliana
2.3.2.15 phytochelatin synthase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.15 35
-
 activity assay Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.15 8
-
 activity assay Arabidopsis thaliana