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Literature summary extracted from
- Enzymatic mechanism and biochemistry for cyanide degradation: A review (2010), J. Hazard. Mater., 176, 1-13.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.9 | L-asparagine | - |
Priestia megaterium |  |
| 4.4.1.9 | L-cysteine | - |
Priestia megaterium | |
| 4.4.1.9 | L-serine | - |
Priestia megaterium | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.5.4 | degradation | this enzyme is of interest for the use in the biodegradation of cyanide and the degradation of nitrile wastes | Escherichia coli |
| 3.5.5.4 | degradation | this enzyme is of interest for the use in the biodegradation of cyanide and the degradation of nitrile wastes | Priestia megaterium |
| 3.5.5.4 | degradation | this enzyme is of interest for the use in the biodegradation of cyanide and the degradation of nitrile wastes | Chromobacterium violaceum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.66 | additional information | EDTA, ammonium chloride, potassium chloride, magnesium chloride or calcium chloride do not inhibit the enzyme activity | Stemphylium loti |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.66 | 300000 | - |
- |
Fusarium solani |
| 4.2.1.66 | 300000 | - |
- |
Fusarium oxysporum |
| 4.2.1.66 | 300000 | - |
- |
Stemphylium loti |
| 4.2.1.66 | 300000 | - |
- |
Microdochium sorghi |
| 4.2.1.66 | 300000 | - |
- |
Exserohilum turcicum |
| 4.2.1.104 | 17008 | - |
x * 17008, calculated from amino acid sequence | Pseudomonas putida |
| 4.2.1.104 | 17008 | - |
x * 17008, calculated from amino acid sequence | Pseudomonas oleovorans |
| 4.4.1.9 | 52000 | - |
- |
Priestia megaterium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.66 | HCN + H2O | Fusarium solani | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Fusarium oxysporum | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Stemphylium loti | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Microdochium sorghi | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Exserohilum turcicum | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Fusarium lateritium | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | Fusarium oxysporum CCMI 876 | HCN is the most effective substrate | formamide | - |
r | |
| 4.2.1.104 | NCO- + HCO3- + H+ | Pseudomonas putida | - |
NH3 + CO2 | - |
? | |
| 4.2.1.104 | NCO- + HCO3- + H+ | Pseudomonas oleovorans | - |
NH3 + CO2 | - |
? | |
| 4.4.1.9 | L-cysteine + HCN | Priestia megaterium | - |
H2S + L-3-cyanoalanine | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.4 | Chromobacterium violaceum | - |
- |
- |
| 3.5.5.4 | Escherichia coli | - |
- |
- |
| 3.5.5.4 | Priestia megaterium | - |
- |
- |
| 4.2.1.66 | Exserohilum turcicum | - |
- |
- |
| 4.2.1.66 | Fusarium lateritium | - |
- |
- |
| 4.2.1.66 | Fusarium oxysporum | - |
- |
- |
| 4.2.1.66 | Fusarium oxysporum CCMI 876 | - |
- |
- |
| 4.2.1.66 | Fusarium solani | - |
- |
- |
| 4.2.1.66 | Microdochium sorghi | - |
- |
- |
| 4.2.1.66 | Stemphylium loti | - |
- |
- |
| 4.2.1.104 | Pseudomonas oleovorans | - |
- |
- |
| 4.2.1.104 | Pseudomonas putida | - |
- |
- |
| 4.4.1.9 | Priestia megaterium | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.66 | 4°C, 4 days, loss of 50% activity | Stemphylium loti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.4 | beta-cyano-L-alanine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
| 3.5.5.4 | beta-cyano-L-alanine + H2O | - |
Priestia megaterium | L-aspartate + NH3 | - |
? | |
| 3.5.5.4 | beta-cyano-L-alanine + H2O | - |
Chromobacterium violaceum | L-aspartate + NH3 | - |
? | |
| 4.2.1.66 | acrylonitrile + H2O | - |
Fusarium oxysporum | acrylamide | - |
? | |
| 4.2.1.66 | acrylonitrile + H2O | - |
Fusarium oxysporum CCMI 876 | acrylamide | - |
? | |
| 4.2.1.66 | crotononitrile + H2O | - |
Fusarium oxysporum | crotonic acid amide | - |
? | |
| 4.2.1.66 | crotononitrile + H2O | - |
Fusarium oxysporum CCMI 876 | crotonic acid amide | - |
? | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Fusarium solani | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Fusarium oxysporum | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Stemphylium loti | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Microdochium sorghi | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Exserohilum turcicum | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Fusarium lateritium | formamide | - |
r | |
| 4.2.1.66 | HCN + H2O | HCN is the most effective substrate | Fusarium oxysporum CCMI 876 | formamide | - |
r | |
| 4.2.1.66 | methacrylonitrile + H2O | - |
Fusarium oxysporum | 2-methylprop-2-enamide | - |
? | |
| 4.2.1.66 | methacrylonitrile + H2O | - |
Fusarium oxysporum CCMI 876 | 2-methylprop-2-enamide | - |
? | |
| 4.2.1.66 | tetracyanonickelate II + H2O | - |
Fusarium oxysporum | ? | - |
? | |
| 4.2.1.66 | tetracyanonickelate II + H2O | - |
Fusarium oxysporum CCMI 876 | ? | - |
? | |
| 4.2.1.104 | NCO- + HCO3- + H+ | - |
Pseudomonas putida | NH3 + CO2 | - |
? | |
| 4.2.1.104 | NCO- + HCO3- + H+ | - |
Pseudomonas oleovorans | NH3 + CO2 | - |
? | |
| 4.4.1.9 | L-cysteine + HCN | - |
Priestia megaterium | H2S + L-3-cyanoalanine | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.104 | ? | x * 17008, calculated from amino acid sequence | Pseudomonas putida |
| 4.2.1.104 | ? | x * 17008, calculated from amino acid sequence | Pseudomonas oleovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.5.4 | beta-cyanoalanine synthase | - |
Escherichia coli |
| 3.5.5.4 | beta-cyanoalanine synthase | - |
Priestia megaterium |
| 3.5.5.4 | beta-cyanoalanine synthase | - |
Chromobacterium violaceum |
| 4.2.1.66 | formamide dehydratase | - |
Fusarium solani |
| 4.2.1.66 | formamide dehydratase | - |
Fusarium oxysporum |
| 4.2.1.66 | formamide dehydratase | - |
Stemphylium loti |
| 4.2.1.66 | formamide dehydratase | - |
Microdochium sorghi |
| 4.2.1.66 | formamide dehydratase | - |
Exserohilum turcicum |
| 4.2.1.66 | formamide dehydratase | - |
Fusarium lateritium |
| 4.2.1.66 | formamide hydro-lyase | - |
Fusarium solani |
| 4.2.1.66 | formamide hydro-lyase | - |
Fusarium oxysporum |
| 4.2.1.66 | formamide hydro-lyase | - |
Stemphylium loti |
| 4.2.1.66 | formamide hydro-lyase | - |
Microdochium sorghi |
| 4.2.1.66 | formamide hydro-lyase | - |
Exserohilum turcicum |
| 4.2.1.66 | formamide hydro-lyase | - |
Fusarium lateritium |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Fusarium solani |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Fusarium oxysporum |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Stemphylium loti |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Microdochium sorghi |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Exserohilum turcicum |
| 4.2.1.66 | formamide hydro-lyase (cyanide-forming) | - |
Fusarium lateritium |
| 4.4.1.9 | beta-cyano-L-alanine synthase | - |
Priestia megaterium |
| 4.4.1.9 | beta-cyanoalanine synthase | - |
Priestia megaterium |
| 4.4.1.9 | beta-cyanoalanine synthetase | - |
Priestia megaterium |
| 4.4.1.9 | L-cysteine hydrogen-sulfide-lyase | - |
Priestia megaterium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.66 | 22 | 24 | the activity is totally lost by incubation of the mycelia for 16 h at 22-24°C | Microdochium sorghi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.66 | 4 | 7 | - |
Fusarium oxysporum |
| 4.2.1.66 | 5.3 | 5.7 | - |
Microdochium sorghi |
| 4.2.1.66 | 6.5 | 7.5 | immobilized enzyme | Stemphylium loti |
| 4.2.1.66 | 7 | 9 | free enzyme | Stemphylium loti |
| 4.2.1.66 | 9 | 10.7 | - |
Fusarium solani |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.9 | pyridoxal 5'-phosphate | contains 1 mol of pyridoxal 5'-phosphate per mol of protein | Priestia megaterium | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.4.1.9 | Priestia megaterium | isoelectric focusing | - |
4.7 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 4.2.1.104 | Pseudomonas putida | cyanase activity is induced during growth with cyanide or cyanate, but not with ammonium or nitrate as the nitrogen source | up |
| 4.2.1.104 | Pseudomonas oleovorans | cyanase activity is induced during growth with cyanide or cyanate, but not with ammonium or nitrate as the nitrogen source | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Fusarium solani |
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Fusarium oxysporum |
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Stemphylium loti |
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Microdochium sorghi |
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Exserohilum turcicum |
| 4.2.1.66 | metabolism | this enzyme participates in cyanoamino acid metabolism | Fusarium lateritium |
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