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Literature summary extracted from
- Purification, characterization, and gene cloning of glucose-1-phosphatase from Citrobacter braakii (2009), J. Gen. Appl. Microbiol., 55, 345-350.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.10 | 0.5 | - |
D-glucose 6-phosphate | Vmax: 0.8 micromol/min/mg | Citrobacter braakii |  |
| 3.1.3.10 | 1.9 | - |
D-glucose 6-phosphate | Vmax: 2.2 micromol/min/mg | Citrobacter braakii | |
| 3.1.3.10 | 5.12 | - |
D-glucose 1-phosphate | Vmax: 1.9 micromol/min/mg | Citrobacter braakii | |
| 3.1.3.10 | 9 | - |
D-mannose 6-phosphate | Vmax: 2.6 micromol/min/mg | Citrobacter braakii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 43710 | - |
calculated from cDNA | Citrobacter braakii |
| 3.1.3.10 | 46000 | - |
SDS-PAGE | Citrobacter braakii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.10 | Citrobacter braakii | D3GGN3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.10 | (NH4)2SO4 precipitation, DEAE-sepharose chromatography, CM-sepharose chromatography and FPLC chromatography | Citrobacter braakii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.10 | D-fructose 6-phosphate + H2O | - |
Citrobacter braakii | D-fructose + phosphate | - |
? | |
| 3.1.3.10 | D-glucose 1-phosphate + H2O | - |
Citrobacter braakii | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | D-glucose 6-phosphate + H2O | - |
Citrobacter braakii | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | D-mannose 6-phosphate + H2O | - |
Citrobacter braakii | D-mannose + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.10 | agpC | - |
Citrobacter braakii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 45 | - |
assay at and optimal temperature | Citrobacter braakii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 55 | - |
enzyme ist stable up to 55°C 30 min | Citrobacter braakii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 5 | - |
assay at and optimal pH | Citrobacter braakii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 3.5 | 7.5 | - |
Citrobacter braakii |
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Release 2023.1 (January 2023)
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