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Literature summary extracted from

  • Zhang, H.; Mao, X.; Wang, Y.; Hu, X.
    Optimization of culture conditions for high-level expression of dextransucrase in Escherichia coli (2009), J. Food Agric. Environ., 7, 75-78.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dexYG, expression in Escherichia coli strain BL21(DE3) Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information optimization of culture conditions for high-level lactose-inducible expression of Leuconostoc mesenteroides dextransucrase in recombinant Escherichia coli strain BL 21(DE3), overview. Maximal activity of 60.18 U/ml from a fed-batch culture at 5 g/l lactose, added at an OD600 of 3.0, at 25°C for 7 h Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 CaCl2 inhibits at 0.1 mM by 64% Leuconostoc mesenteroides
2.4.1.5 FeSO4 inhibits 26.5% at 0.1 mM, but activates 39.5% at 0.05 mM Leuconostoc mesenteroides
2.4.1.5 MnSO4 activates 68% at 0.1 mM, but inhibits at 0.05 mM by 26% Leuconostoc mesenteroides
2.4.1.5 ZnSO4 inhibits 27% at 0.05 mM and 24.5% at 0.1 mM Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 FeSO4 inhibits 26.5% at 0.1 mM, but activates 39.5% at 0.05 mM Leuconostoc mesenteroides
2.4.1.5 MgSO4 activates 115.6% at 0.1 mM, 20% at 0.05 mM Leuconostoc mesenteroides
2.4.1.5 MnSO4 activates 68% at 0.1 mM, but inhibits at 0.05 mM by 26% Leuconostoc mesenteroides
2.4.1.5 NaCl activates 11% at 0.1 mM Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 gene dexYG
-
2.4.1.5 Leuconostoc mesenteroides 0326
-
 gene dexYG
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 25
-
 assay at Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
 assay at Leuconostoc mesenteroides