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Literature summary extracted from

  • Ui, S.; Okajima, Y.; Mimura, A.; Kanai, H.; kobayashi, T.; Kudo, T.
    Sequence analysis of the gene for and characterization of D-acetoin forming meso-2,3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli (1997), J. Ferment. Bioeng., 83, 32-37.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.304 expression in Escherichia coli Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.304 26591
-
 4 * 26591, calculated Klebsiella pneumoniae
1.1.1.304 96000
-
 gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.304 Klebsiella pneumoniae Q48436 bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.304 2,3-pentanedione + NADH + H+ 77% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin Klebsiella pneumoniae 3-hydroxy-2-pentanone + NAD+
-
 ?
1.1.1.304 diacetyl + NADH + H+ 87% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin Klebsiella pneumoniae (S)-acetoin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.304 tetramer 4 * 26591, calculated Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.304 7 8
-
 Klebsiella pneumoniae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.304 Klebsiella pneumoniae isoelectric focusing 7.2 5.9