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Literature summary extracted from

  • Clark, S.M.; Di Leo, R.; Van Cauwenberghe, O.R.; Mullen, R.T.; Shelp, B.J.
    Subcellular localization and expression of multiple tomato gamma-aminobutyrate transaminases that utilize both pyruvate and glyoxylate (2009), J. Exp. Bot., 60, 3255-3267.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.19 full-length GABA-T1 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T1 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells Solanum lycopersicum
2.6.1.19 full-length GABA-T2 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T2 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells Solanum lycopersicum
2.6.1.19 full-length GABA-T3 isoform fused to the green fluorescent protein is expressed in Nicotiana tabacum, and GABA-T3 isoform is co-expressed with the GroES/EL molecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells Solanum lycopersicum
2.6.1.96 isozyme GABA-T1, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T1 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum
2.6.1.96 isozyme GABA-T2, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum
2.6.1.96 isozyme GABA-T3, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T3 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 mitochondrion isoform GABA-T1 Solanum lycopersicum 5739
-
2.6.1.96 mitochondrion
-
 Solanum lycopersicum 5739
-
2.6.1.96 additional information the subcellular distribution of GABA-T in the plant kingdom is highly variable, in silico analysis of GABA-T isozyme subcellular localizations in tomato, overview Solanum lycopersicum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.96 4-aminobutanoate + glyoxylate Solanum lycopersicum
-
 succinate semialdehyde + glycine
-
 ?
2.6.1.96 4-aminobutanoate + pyruvate Solanum lycopersicum
-
 succinate semialdehyde + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Solanum lycopersicum Q84P52
-
-
2.6.1.19 Solanum lycopersicum Q84P53
-
-
2.6.1.19 Solanum lycopersicum Q84P54
-
-
2.6.1.96 Solanum lycopersicum Q84P52 isozyme GABA-T3; contains three GABA-T isoforms
-
2.6.1.96 Solanum lycopersicum Q84P53 isozyme GABA-T2; contains three GABA-T isoforms
-
2.6.1.96 Solanum lycopersicum Q84P54 isozyme GABA-T1; contains three GABA-T isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.19
-
 Solanum lycopersicum
2.6.1.96 recombinant His-tagged isozyme GABA-T1 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum
2.6.1.96 recombinant His-tagged isozyme GABA-T2 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum
2.6.1.96 recombinant His-tagged isozyme GABA-T3 from Escherichia coli strain BL-21(DE3) Rosetta (pLysS) by nickel affinity chromatgraphy Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.19 carpel
-
 Solanum lycopersicum
-
2.6.1.19 fruit
-
 Solanum lycopersicum
-
2.6.1.19 fruit highest expression in ripe fruit leaf at day 92 Solanum lycopersicum
-
2.6.1.19 fruit highest expression of in ripe fruit leaf at day 92 Solanum lycopersicum
-
2.6.1.19 leaf
-
 Solanum lycopersicum
-
2.6.1.19 petal
-
 Solanum lycopersicum
-
2.6.1.19 root
-
 Solanum lycopersicum
-
2.6.1.19 stamen
-
 Solanum lycopersicum
-
2.6.1.19 stem
-
 Solanum lycopersicum
-
2.6.1.96 carpel
-
 Solanum lycopersicum
-
2.6.1.96 fruit
-
 Solanum lycopersicum
-
2.6.1.96 fruit GABA-T1 is the predominant isoform in stamens and maturing fruit tissue Solanum lycopersicum
-
2.6.1.96 leaf
-
 Solanum lycopersicum
-
2.6.1.96 additional information differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, overview Solanum lycopersicum
-
2.6.1.96 petal
-
 Solanum lycopersicum
-
2.6.1.96 root
-
 Solanum lycopersicum
-
2.6.1.96 sepal
-
 Solanum lycopersicum
-
2.6.1.96 stamen
-
 Solanum lycopersicum
-
2.6.1.96 stem
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.19 0.0183
-
 isoform GABA-T2, using pyruvate as amino cceptor Solanum lycopersicum
2.6.1.19 0.0209
-
 isoform GABA-T2, using glyoxylate as amino cceptor Solanum lycopersicum
2.6.1.19 0.1785
-
 isoform GABA-T3, using glyoxylate as amino acceptor Solanum lycopersicum
2.6.1.19 0.2065
-
 isoform GABA-T3, using pyruvate as amino acceptor Solanum lycopersicum
2.6.1.19 3.495
-
 isoform GABA-T1, using glyoxylate as amino acceptor Solanum lycopersicum
2.6.1.19 5.79
-
 isoform GABA-T1, using pyruvate as amino acceptor Solanum lycopersicum
2.6.1.96 0.0183
-
 purified recombinant isozyme GABA-T2, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum
2.6.1.96 0.0209
-
 purified recombinant isozyme GABA-T2, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
2.6.1.96 0.179
-
 purified recombinant isozyme GABA-T3, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
2.6.1.96 0.207
-
 purified recombinant isozyme GABA-T3, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum
2.6.1.96 3.5
-
 purified recombinant isozyme GABA-T1, with glyoxylate as substrate, pH 9.0, 30°C Solanum lycopersicum
2.6.1.96 5.79
-
 purified recombinant isozyme GABA-T1, with pyruvate as substrate, pH 9.0, 30°C Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + glyoxylate
-
 Solanum lycopersicum 4-oxobutanoate + glycine
-
 ?
2.6.1.19 4-aminobutanoate + pyruvate
-
 Solanum lycopersicum 4-oxobutanoate + L-alanine
-
 ?
2.6.1.96 4-aminobutanoate + glyoxylate
-
 Solanum lycopersicum succinate semialdehyde + glycine
-
 ?
2.6.1.96 4-aminobutanoate + pyruvate
-
 Solanum lycopersicum succinate semialdehyde + L-alanine
-
 ?
2.6.1.96 additional information GABA-T isozymes catalyse the breakdown of GABA to succinic semialdehyde. The isozymes all have both pyruvate- and glyoxylate-dependent activities, and utilize GABA and Ala but not beta-Ala, Orn, acetylornithine, Ser, Gly, Asn, Gln, Glu, Val, Leu, Ile, Met, Phe, His, Lys, Arg, Asp, Thr, Tyr, Trp, Pro, or Cys as amino donors Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.19 GABA-T1 isoform Solanum lycopersicum
2.6.1.19 GABA-T2 isoform Solanum lycopersicum
2.6.1.19 GABA-T3 isoform Solanum lycopersicum
2.6.1.19 gamma-aminobutyrate transaminases
-
 Solanum lycopersicum
2.6.1.19 gamma-aminobutyric acid transaminase
-
 Solanum lycopersicum
2.6.1.96 GABA-T
-
 Solanum lycopersicum
2.6.1.96 gamma-aminobutyrate transaminase
-
 Solanum lycopersicum
2.6.1.96 gamma-aminobutyric acid transaminase
-
 Solanum lycopersicum
2.6.1.96 gammaaAminobutyric acid transaminase
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.96 30
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.96 9
-
 assay at Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.19 pyridoxal 5'-phosphate
-
 Solanum lycopersicum
2.6.1.96 pyridoxal 5'-phosphate
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
2.6.1.19 physiological function isoform GABA-T1 plays the predominant role in GABA metabolism in vegetative tissue Solanum lycopersicum
2.6.1.96 physiological function unique roles for each isozyme in developmental processes Solanum lycopersicum