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Literature summary extracted from

  • Schriek, S.; Kahmann, U.; Staiger, D.; Pistorius, E.K.; Michel, K.P.
    Detection of an L-amino acid dehydrogenase activity in Synechocystis sp. PCC 6803 (2009), J. Exp. Bot., 60, 1035-1046.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.5 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone 0.03 mM, 50% inhibition Synechocystis sp.
1.4.1.5 CaCl2 0.09 mM, 50% inhibition Synechocystis sp.
1.4.1.5 CoCl2 0.11 mM, 50% inhibition Synechocystis sp.
1.4.1.5 dipyridyl 0.01 mM, 50% inhibition Synechocystis sp.
1.4.1.5 KCN 0.1 mM, 50% inhibition Synechocystis sp.
1.4.1.5 MgCl2 2 mM inhibits enzymatic activity by 50%, 10 mM MgCl2 completely inhibits enzymatic activity Synechocystis sp.
1.4.1.5 NaCl 8 mM, 50% inhibition Synechocystis sp.
1.4.1.5 NaN3 0.1 mM, 50% inhibition Synechocystis sp.
1.4.1.5 NiCl2 0.09 mM, 50% inhibition Synechocystis sp.
1.4.1.5 o-phenanthroline 0.05 mM, 50% inhibition Synechocystis sp.
1.4.1.5 Salicylhydroxamic acid 0.02 mM, 50% inhibition Synechocystis sp.
1.4.1.5 SrCl2 0.13 mM, 50% inhibition Synechocystis sp.
1.4.1.5 ZnCl2 0.2 mM, 50% inhibition Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.5 3.2
-
 L-arginine pH 8.5 Synechocystis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.5 thylakoid membrane
-
 Synechocystis sp. 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.5 MgCl2 0.5 mM stimulate activity by 1.7fold Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.5 51400
-
 deduced from amino acid sequence Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.5 Synechocystis sp. Q55629 PCC 6803
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.5 glycine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-alanine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-arginine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-cysteine + H2O + NAD+ best substrate besides L-arginine Synechocystis sp. 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-glutamate + H2O + NAD+
-
 Synechocystis sp. 2-oxoglutarate + NH3 + NADH
-
 ?
1.4.1.5 L-glutamine + H2O + NAD+
-
 Synechocystis sp. 2-oxoglutaramate + NH3 + NADH
-
 ?
1.4.1.5 L-histidine + H2O + NAD+
-
 Synechocystis sp. 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-isoleucine + H2O + NAD+
-
 Synechocystis sp. 3-methyl-2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.5 L-leucine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-Lysine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-methionine + H2O + NAD+
-
 Synechocystis sp. (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.5 L-ornithine + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-phenylalanine + H2O + NAD+
-
 Synechocystis sp. phenylpyruvate + NH3 + NADH
-
 ?
1.4.1.5 L-proline + H2O + NAD+
-
 Synechocystis sp. ? + NH3 + NADH
-
 ?
1.4.1.5 L-threonine + H2O + NAD+
-
 Synechocystis sp. 3-hydroxy-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-tryptophan + H2O + NAD+
-
 Synechocystis sp. 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-tyrosine + H2O + NAD+
-
 Synechocystis sp. (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
 ?
1.4.1.5 L-valine + H2O + NAD+
-
 Synechocystis sp. 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.5 additional information utilizing toluene-permeabilized cells, an L-amino acid- stimulated O2 uptake became detectable in Synechocystis sp. PCC 6803 in a Clark-type electrode. Enzyme reacts specifically with L-amino acids D-amino acids show no reactivity Synechocystis sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.5 L-amino acid dehydrogenase
-
 Synechocystis sp.
1.4.1.5 Slr0782
-
 Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.5 8.5
-
 assay at Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.5 FAD
-
 Synechocystis sp.

Expression

EC Number Organism Comment Expression
1.4.1.5 Synechocystis sp. slot-blot RNA-DNA hybridization experiments show that the slr0782 mRNA level increases, when cells are grown with L-arginine as compared to the growth of cells with nitrate up