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Literature summary extracted from

  • Capuder, M.; Solar, T.; Bencina, M.; Legisa, M.
    Highly active, citrate inhibition resistant form of Aspergillus niger 6-phosphofructo-1-kinase encoded by a modified pfkA gene (2009), J. Biotechnol., 144, 51-57.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 D-fructose 2,6-bisphosphate the active 49 kDa PFK1 fragment is activated to a higher level by D-fructose 2,6-bisphosphate with respect to the native enzyme, a significant boost in the enzyme activity of the fragment is registered at 1 mM ATP after the introduction of 0.004 mM of D-fructose-2,6-bisphosphate Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP in contrast to the native PFK1 enzyme, the 49 kDa PFK1 fragment is highly sensitive to ATP inhibition (5fold lower activity at the 1 mM ATP than at the 0.1 mM concentration) Aspergillus niger
2.7.1.11 citrate when 5 mM of citrate is added a moderate reduction for about 10–20% is recorded in the homogenate of TE22 and TE23 strain, while in A158 strain much stronger reduction of PFK1 specific activity, for approximately 40% is observed Aspergillus niger
2.7.1.11 additional information the active 49 kDa PFK1 fragment is not inhibited by citrate Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 85000
-
 native enzyme, gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Aspergillus niger P78985 strains A158, TE22, and TE23
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.11 phosphoprotein in Aspergillus niger cells spontaneous posttranslational modification of 6-phosphofructo-1-kinase occurs in a two step process. The native enzyme (85 kDa) is first cleaved to an inactive fragment (49 kDa) that regains its activity after phosphorylation of the protein, the shorter fragment exhibits changed kinetics, such as resistance to citrate inhibition Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aspergillus niger ADP + D-fructose 1,6-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 6-phosphofructo-1-kinase
-
 Aspergillus niger
2.7.1.11 PFK1
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.11 ATP
-
 Aspergillus niger