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Literature summary extracted from

  • Nishimura, H.; Sako, Y.
    Purification and characterization of the oxygen-thermostable hydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum camini (2009), J. Biosci. Bioeng., 108, 299-303.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 O2 maintains 100% of its initial activity after a 48 h exposure to air, after 120 and 168 h exposures, the residual activities are 90% and 75%, respectively. It is very tolerant to oxygen Aeropyrum camini
1.12.99.6 O2 exhibits 80% residual activity after exposure to air for 24 h, decreases to 75% and 37% after 48 and 72 h exposures, respectively. After a 96 h exposure, activity is undetectable. The enzyme is oxygen sensitive Persephonella hydrogeniphila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 soluble
-
 Aeropyrum camini
-
-
1.12.99.6 soluble
-
 Persephonella hydrogeniphila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 85000
-
 x * 85000, SDS-PAGE Aeropyrum camini

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Aeropyrum camini
-
-
-
1.12.99.6 Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091
-
-
-
1.12.99.6 Persephonella hydrogeniphila
-
-
-
1.12.99.6 Persephonella hydrogeniphila 29W / DSM 15103 / JCM11663
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 by gel filtration, 55fold Persephonella hydrogeniphila
1.12.99.6 to homogeneity by gel filtration, 2.4fold Aeropyrum camini

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 14.6
-
 purified enzyme, proton reduction activity Aeropyrum camini
1.12.99.6 14.8
-
 2.4fold purified enzyme, hydrogen oxidation activity Aeropyrum camini
1.12.99.6 50
-
-
 Persephonella hydrogeniphila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized methyl viologen
-
 Aeropyrum camini H+ + reduced methyl viologen
-
 ?
1.12.99.6 H2 + oxidized methyl viologen
-
 Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091 H+ + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 ? x * 85000, SDS-PAGE Aeropyrum camini

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 hydrogenase II
-
 Aeropyrum camini

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.99.6 85
-
 optimal temperature for the hydrogen oxidation activity and for the proton reduction activity Aeropyrum camini

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 90
-
 half-life of 48 h, shows hyperthermophily Aeropyrum camini
1.12.99.6 90
-
 shows complete denaturation at 90°C after incubation for 30 min Persephonella hydrogeniphila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 6.5
-
 optimal pH for the proton reduction activity Aeropyrum camini
1.12.99.6 8.5
-
 optimal pH for the hydrogen oxidation activity Aeropyrum camini

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.99.6 6 9 optimal pH range for the hydrogen oxidation activity is 8.5 to 9.0, optimal pH range for the proton reduction activity is 6.0 to 6.5 Aeropyrum camini

General Information

EC Number General Information Comment Organism
1.12.99.6 physiological function functions as a sensory hydrogenase Aeropyrum camini