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Literature summary extracted from
- Microbial production of xylitol from L-arabinose by metabolically engineered Escherichia coli (2009), J. Biosci. Bioeng., 107, 506-511.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | enzyme coexpression in Escherichia coli strain ZUC99 with D-psicose 3-epimerase and L-xylulose reductase, leading to production of L-xylitol, under control of the araBAD promoter | Escherichia coli K-12 |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | Mn2+ | required | Escherichia coli K-12 |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | L-arabinose | Escherichia coli K-12 | - |
L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | Escherichia coli K-12 AB707 | - |
L-ribulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.4 | Escherichia coli K-12 | - |
gene araA | - |
| 5.3.1.4 | Escherichia coli K-12 AB707 | - |
gene araA | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | L-arabinose | - |
Escherichia coli K-12 | L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Escherichia coli K-12 AB707 | L-ribulose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | AraA | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 30 | - |
assay at | Escherichia coli K-12 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 7.5 | - |
assay at | Escherichia coli K-12 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 5.3.1.4 | Escherichia coli K-12 | production of xylitol from L-arabinose by metabolically engineered Escherichia coli enzyme expressing three enzymes in strain ZUC99: L-arabinose isomerase, which converts L-arabinose to L-ribulose, D-psicose 3-epimerase, which converts L-ribulose to L-xylulose, and L-xylulose reductase, which converts L-xylulose to xylitol, method optimization, overview | additional information |
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Release 2023.1 (January 2023)
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