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Literature summary extracted from

  • Ju, J.; Xu, S.; Wen, J.; Li, G.; Ohnishi, K.; Xue, Y.; Ma, Y.
    Characterization of endogenous pyridoxal 5-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4 (2009), J. Biosci. Bioeng., 107, 225-229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 subcloned in a D-alanine auxotrophic Escherichia coli strain MB2795 and in Escherichia coli BL21(DE3) Alkalihalophilus pseudofirmus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 14.91
-
 D-alanine
-
 Alkalihalophilus pseudofirmus
5.1.1.1 41.79
-
 L-alanine
-
 Alkalihalophilus pseudofirmus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.1 exosporium
-
 Alkalihalophilus pseudofirmus 43592
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 41000
-
 SDS-PAGE Alkalihalophilus pseudofirmus
5.1.1.1 87000
-
 gel filtration Alkalihalophilus pseudofirmus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Alkalihalophilus pseudofirmus B3VI72
-
-
5.1.1.1 Alkalihalophilus pseudofirmus OF4 B3VI72
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Alkalihalophilus pseudofirmus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.1.1 spore
-
 Alkalihalophilus pseudofirmus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 3708
-
 substrate is D-alanine Alkalihalophilus pseudofirmus
5.1.1.1 10500
-
 substrate is L-alanine Alkalihalophilus pseudofirmus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-alanine
-
 Alkalihalophilus pseudofirmus D-alanine
-
 r
5.1.1.1 L-alanine
-
 Alkalihalophilus pseudofirmus OF4 D-alanine
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 41000, gel filtration and dynamic light scattering Alkalihalophilus pseudofirmus

Synonyms

EC Number Synonyms Comment Organism
5.1.1.1 alanine racemase
-
 Alkalihalophilus pseudofirmus
5.1.1.1 dadXOF4
-
 Alkalihalophilus pseudofirmus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 40
-
-
 Alkalihalophilus pseudofirmus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.1.1
-
 70 between 20 to 50°C the enzyme activity is 75-100%, at 0°C activity is about 28% and at 70°C activity is 20% Alkalihalophilus pseudofirmus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 10.5
-
-
 Alkalihalophilus pseudofirmus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.1.1 7.5 12.5 at pH 7.5 and at pH 12.5 activity is just under 40%, at pH 7 and at pH 13 the enzyme is inactive Alkalihalophilus pseudofirmus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate PLP is bound to the enzyme, adding PLP during developement of enzyme or to an assay is not necessary Alkalihalophilus pseudofirmus