BRENDA - Enzyme Database

Enhancement of over expression and chaperone assisted yield of folded recombinant aconitase in Escherichia coli in bioreactor cultures

Gupta, P.; Ghosalkar, A.; Mishra, S.; Chaudhuri, T.K.; J. Biosci. Bioeng. 107, 102-107 (2009)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.3
biotechnology
molecular chaperones GroEL/GroES are co-expressed with soluble, biologically active recombinant aconitase in Escherichia coli by cultivation in a bioreactor at different temperatures under optimized conditions. The yield of functional aconitase is enhanced, either in presence of co-expressed GroEL/ES or at low temperature cultivation. The outcome from the chaperone assisted folding of aconitase is more pronounced at lower temperature
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.3
yeast mitochondrial aconitase is expressed in Escherichia coli by cultivation in a bioreactor at different temperatures. Chaperones GroEL and GroES are co-expressed
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
using column chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
704735
Saccharomyces cerevisiae
cis-aconitate + H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
assay at
Saccharomyces cerevisiae
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.3
biotechnology
molecular chaperones GroEL/GroES are co-expressed with soluble, biologically active recombinant aconitase in Escherichia coli by cultivation in a bioreactor at different temperatures under optimized conditions. The yield of functional aconitase is enhanced, either in presence of co-expressed GroEL/ES or at low temperature cultivation. The outcome from the chaperone assisted folding of aconitase is more pronounced at lower temperature
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
yeast mitochondrial aconitase is expressed in Escherichia coli by cultivation in a bioreactor at different temperatures. Chaperones GroEL and GroES are co-expressed
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
using column chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
704735
Saccharomyces cerevisiae
cis-aconitate + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
assay at
Saccharomyces cerevisiae