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Literature summary extracted from

  • Fujisawa, M.; Watanabe, M.; Choi, S.K.; Teramoto, M.; Ohyama, K.; Misawa, N.
    Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB (2008), J. Biosci. Bioeng., 105, 636-641.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.32 biotechnology developement nutritional plants enriched with carotenoids Pantoea ananatis
2.5.1.32 food industry developement nutritional plants enriched with carotenoids Pantoea ananatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.199 expressed in Arabidopsis thaliana and Brassica carinata Crambe hispanica subsp. abyssinica
2.5.1.32 expressed under the control of the cauliflower mosaic virus 35S constitutive promoter or the Arabidopsis thaliana fatty acid elongase 1 gene seed-specific promoter in linseed flax Linum usitatissimum after transformation with Agrobacterium tumefaciens, in both cases transcripts are only detectable in seeds but not in leaves Pantoea ananatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.199 56400
-
 calculated from amino acid sequence Crambe hispanica subsp. abyssinica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.199 Crambe hispanica subsp. abyssinica Q32VC2
-
-
2.5.1.32 Pantoea ananatis
-
 formerly called Erwinia uredovora 20D3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.199 eicosenoyl-CoA + malonyl-CoA 22:1DELTA13 Crambe hispanica subsp. abyssinica ?
-
 ?
2.3.1.199 erucoyl-CoA + malonyl-CoA 20:1DELTA11 Crambe hispanica subsp. abyssinica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.199 3-ketoacyl-CoA synthase
-
 Crambe hispanica subsp. abyssinica
2.3.1.199 FAE1
-
 Crambe hispanica subsp. abyssinica
2.3.1.199 fatty acid elongation 1
-
 Crambe hispanica subsp. abyssinica
2.3.1.199 KCS
-
 Crambe hispanica subsp. abyssinica
2.5.1.32 CrtB
-
 Pantoea ananatis
2.5.1.32 phytoene synthase
-
 Pantoea ananatis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.199 Crambe hispanica subsp. abyssinica calculated from amino acid sequence
-
 9.3

General Information

EC Number General Information Comment Organism
2.3.1.199 physiological function the enzyme has strong capability for improving the erucic acid content Crambe hispanica subsp. abyssinica
2.5.1.32 metabolism first step in the biosynthesis of alpha-carotene, beta-carotene, and lutein Pantoea ananatis