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Literature summary extracted from

  • Yu, X.C.; Miranda, M.; Liu, Z.; Patel, S.; Nguyen, N.; Carson, K.; Liu, Q.; Swaffield, J.C.
    Novel potent inhibitors of deoxycytidine kinase identified and compared by multiple assays (2010), J. Biomol. Screen., 15, 72-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 LP-375752 i.e. 2-[4-(6-benzo[b]thiophen-2-yl-pyrimidin-4-yl)-piperazin-1-yl]-1-pyrrolidin-1-yl-ethanone Homo sapiens
2.7.1.74 LP-503392 i.e. 2-thio-2'-deoxycytidine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.001
-
 2'-deoxycytidine
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 additional information Homo sapiens dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 1-beta-D-arabinosylcytosine
-
 Homo sapiens ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 ATP + cladribine
-
 Homo sapiens ADP + cladribine 5'-phosphate
-
 ?
2.7.1.74 ATP + fludarabine
-
 Homo sapiens ADP + fludarabine 5'-phosphate
-
 ?
2.7.1.74 ATP + gemcitabine
-
 Homo sapiens ADP + gemcitabine 5'-phosphate
-
 ?
2.7.1.74 additional information dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.74 ATP
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.74 0.00091
-
 in 50 mM Tris (pH 7.6) Homo sapiens LP-503392
2.7.1.74 0.0138
-
 in 50 mM Tris (pH 7.6) Homo sapiens LP-375752

General Information

EC Number General Information Comment Organism
2.7.1.74 metabolism dCK plays an important role in the salvage pathway of nucleoside metabolism Homo sapiens