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Literature summary extracted from

  • Kristan, K.; Kotnik, M.; Oblak, M.; Urleb, U.
    New high-throughput fluorimetric assay for discovering inhibitors of UDP-N-acetylmuramyl-L-alanine: D-glutamate (MurD) ligase (2009), J. Biomol. Screen., 14, 412-418.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.9 analysis assay for monitoring enzyme activity based on the accumulation of adenosine 5'-diphosphate, a product of the reaction catalyzed by MurD ligase, by conversion to a fluorescent signal via a coupled enzyme system, with counterscreen assay to eliminate false positive results Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.9
-
Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.9 3-([[(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]acetyl]amino)-4-methylbenzoic acid
-
Escherichia coli
6.3.2.9 beta,gamma-methyleneadenosine 5'-triphosphate nonhydrolyzable ATP analogue Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.9 0.0073
-
UDP-N-acetylmuramate-L-alanine 37°C Escherichia coli
6.3.2.9 0.067
-
D-glutamate 37°C Escherichia coli
6.3.2.9 0.106
-
ATP 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.9 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.9 ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
-
Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.2.9 0.105
-
37°C Escherichia coli 3-([[(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]acetyl]amino)-4-methylbenzoic acid
6.3.2.9 0.177
-
37°C Escherichia coli beta,gamma-methyleneadenosine 5'-triphosphate