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Literature summary extracted from

  • Vodovar, N.; Schweisguth, F.
    Functions of O-fucosyltransferase in Notch trafficking and signaling: Towards the end of a controversy? (2008), J. Biol., 7, 7-7.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.221 R275A is catalytically dead. Ofut1 and mutant both bind the Notch extracellular domain, and expression of the mutant in cultured cells can increase both the amount and the ligand-binding activity of secreted Notch extracellular domain. Complete loss of ofut1 activity results in a strong phenotype mimicking a loss of Notch activity that is rescued to larval viability by the expression of mutant R275A, it rescues Notch receptor activity in these ofut1 mutant cells and leads to phenotypes mimicking a loss of fringe activity Drosophila sp. (in: flies)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.221 cell surface cell surface accumulation of Notch in ofut1 mutant cells Drosophila sp. (in: flies) 9986
-
2.4.1.221 endoplasmic reticulum Ofut1 acts as a chaperone in the endoplasmic reticulum to promote the proper folding of the epidermal growth factor-like repeats of Notch, thereby ensuring its correct cell-surface localization and ligand-binding activity Drosophila sp. (in: flies) 5783
-
2.4.1.221 additional information Ofut1 is required for the proper distribution of Notch at adherens junction Drosophila sp. (in: flies)
-
-
2.4.1.221 soluble
-
 Drosophila sp. (in: flies)
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.221 Drosophila sp. (in: flies)
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.221 GDP-L-fucose + Notch O-fucosylation of Notch, catalytic and non-catalytic activities Drosophila sp. (in: flies) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.221 O-fucosyltransferase
-
 Drosophila sp. (in: flies)
2.4.1.221 OFUT1
-
 Drosophila sp. (in: flies)

General Information

EC Number General Information Comment Organism
2.4.1.221 malfunction in ofut1 mutant cells, Notch goes to the membrane, is internalized and accumulates in an uncharacterized endocytic compartment. Knockdown of Ofut1 using doublestranded RNA in cultured cells inhibits the secretion of a soluble version of the Notch extracellular domain. In ofut1 mutant cells, Notch does not accumulate at adherens junctions but instead accumulates into intracellular dots. A low level of Notch is also present at the surface of ofut1 mutant cells Drosophila sp. (in: flies)
2.4.1.221 physiological function roles in the folding of Notch in the endoplasmic reticulum, in Notch-ligand binding and in endocytic trafficking of Notch. The carboxy-terminal extremity of Ofut1 contains a Lys-Asp-Glu-Leu (KDEL)-like motif that is dispensable for its catalytic activity but is required for both endoplasmic reticulum retention and function. Ofut1 is required only for Fringe-dependent signaling events Drosophila sp. (in: flies)