Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamanaka, Y.; Hashimoto, K.; Ohtaki, A.; Noguchi, K.; Yohda, M.; Odaka, M.
    Kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase (2010), J. Biol. Inorg. Chem., 15, 655-665.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 mutant enzymes are expressed in Escherichia coli HMS174(DE3)pLysS cells Rhodococcus erythropolis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.84 wild type and mutant enzymes S113A and Y72F, hanging drop vapor diffusion method, using 20% (w/v) PEG8000, 0.10 M Tris-HCl, pH 7.5, 0.3 M MgCl2 Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.84 S113A the mutation partially affects catalytic activity and does not change the pH profiles of the kinetic parameters, the electronic state of the Fe center is altered Rhodococcus erythropolis
4.2.1.84 Y72F the mutant exhibits no activity Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 n-butyric acid competitive inhibitor Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.75
-
 Methacrylonitrile wild type enzyme, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis
4.2.1.84 1.3
-
 Methacrylonitrile mutant enzyme S113A, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe3+ has an unusual Fe3+ center with two modified Cys ligands Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus erythropolis
-
-
-
4.2.1.84 Rhodococcus erythropolis N771
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 methacrylonitrile + H2O
-
 Rhodococcus erythropolis methacrylamide
-
 ?
4.2.1.84 methacrylonitrile + H2O
-
 Rhodococcus erythropolis N771 methacrylamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Rhodococcus erythropolis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.84 0.0000065
-
 Methacrylonitrile mutant enzyme S113A, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis
4.2.1.84 0.000016
-
 Methacrylonitrile wild type enzyme, in 50 mM sodium phosphate, pH 7.5, at 22°C Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.84 pyrroloquinoline quinone
-
 Rhodococcus erythropolis