Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Oakley, A.J.; Coggan, M.; Board, P.G.
    Identification and characterization of {gamma}-glutamylamine cyclotransferase: An enzyme responsible for {gamma}-glutamyl-{epsilon}-lysine catabolism (2010), J. Biol. Chem., 285, 9642-9648.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.9 into the vector pGEM-T for sequencing and subsequently into pHUE for expression of the protein in Escherichia coli BL21DE3 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.9 the structures of GGACT, GGACT in complex with 5-oxoproline, and the E82Q mutant are solved to a resolution of 1.20, 1.20 and 0.98 A, respectively Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.9 E82A mutant, inactive Homo sapiens
4.3.2.9 E82Q mutant, inactive Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.9 17300
-
 recombinant, expressed in Escherichia coli Homo sapiens
4.3.2.9 17330
-
 theoretical, human A2LD1 Homo sapiens
4.3.2.9 18000
-
 determined by gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.9 (gamma-L-glutamyl)-L-amino acid Homo sapiens
-
 5-oxoproline + L-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Homo sapiens Q9BVM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9 by Ni-agarose chromatography, subsequent removal of all additional N-terminal residues by cleavage with the catalytic domain of mouse ubiquitin specific protease 2 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.9 2.7
-
 L-gamma-glutamyl-L-epsilon-lysine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 (gamma-L-glutamyl)-L-amino acid
-
 Homo sapiens 5-oxoproline + L-amino acid
-
 ?
4.3.2.9 L-gamma-glutamyl-L-epsilon-lysine
-
 Homo sapiens 5-oxoproline + L-lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.9 monomer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.3.2.9 A2LD1
-
 Homo sapiens
4.3.2.9 GGACT
-
 Homo sapiens
4.3.2.9 {gamma}-glutamylamine cyclotransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.2.9 37
-
 activity assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.9 8
-
 activity assay Homo sapiens