EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.2.9 | into the vector pGEM-T for sequencing and subsequently into pHUE for expression of the protein in Escherichia coli BL21DE3 cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.2.9 | the structures of GGACT, GGACT in complex with 5-oxoproline, and the E82Q mutant are solved to a resolution of 1.20, 1.20 and 0.98 A, respectively | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.2.9 | E82A | mutant, inactive | Homo sapiens |
4.3.2.9 | E82Q | mutant, inactive | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.2.9 | 17300 | - |
recombinant, expressed in Escherichia coli | Homo sapiens |
4.3.2.9 | 17330 | - |
theoretical, human A2LD1 | Homo sapiens |
4.3.2.9 | 18000 | - |
determined by gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.2.9 | (gamma-L-glutamyl)-L-amino acid | Homo sapiens | - |
5-oxoproline + L-amino acid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.2.9 | Homo sapiens | Q9BVM4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.2.9 | by Ni-agarose chromatography, subsequent removal of all additional N-terminal residues by cleavage with the catalytic domain of mouse ubiquitin specific protease 2 | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.2.9 | 2.7 | - |
L-gamma-glutamyl-L-epsilon-lysine | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.2.9 | (gamma-L-glutamyl)-L-amino acid | - |
Homo sapiens | 5-oxoproline + L-amino acid | - |
? | |
4.3.2.9 | L-gamma-glutamyl-L-epsilon-lysine | - |
Homo sapiens | 5-oxoproline + L-lysine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.2.9 | monomer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.2.9 | A2LD1 | - |
Homo sapiens |
4.3.2.9 | GGACT | - |
Homo sapiens |
4.3.2.9 | {gamma}-glutamylamine cyclotransferase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.2.9 | 37 | - |
activity assay | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.2.9 | 8 | - |
activity assay | Homo sapiens |