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Literature summary extracted from

  • Voss, J.E.; Scally, S.W.; Taylor, N.L.; Atkinson, S.C.; Griffin, M.D.; Hutton, C.A.; Parker, M.W.; Alderton, M.R.; Gerrard, J.A.; Dobson, R.C.; Dogovski, C.; Perugini, M.A.
    Substrate-mediated stabilization of a tetrameric drug target reveals Achilles heel in anthrax (2010), J. Biol. Chem., 285, 5188-5195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 dapA gene encoding DHDPS amplified and cloned into the pET11a expression vector, expressed in Escherichia coli BL21-DE3 Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 in complex with pyruvate, by sitting- and hanging-drop vapor diffusion method, to 2.15 A resolution, shares the same space group, unit cell parameters, and a similar resolution to the structure of substrate unbound DHDPS. Twelve more hydrogen bond interactions at both interfaces in the crystal structure of pyruvate-bound DHDPS relative to the apo structure Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.7 L170E/G191E dimeric mutant of the enzyme, retains only 1.8% of the total catalytic activity of the wild-type tetrameric enzyme Bacillus anthracis

General Stability

EC Number General Stability Organism
4.3.3.7 the secondary and quaternary structure is significantly stabilized in the presence of pyruvate Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.7 0.05
-
 L-aspartate 4-semialdehyde mutant L170E/G191E, at 30°C Bacillus anthracis
4.3.3.7 0.25
-
 L-aspartate 4-semialdehyde wild-type, at 30°C Bacillus anthracis
4.3.3.7 1.2
-
 pyruvate wild-type, at 30°C Bacillus anthracis
4.3.3.7 3.7
-
 pyruvate mutant L170E/G191E, at 30°C Bacillus anthracis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.7 31000
-
 2 * 31000, calculated from sequence Bacillus anthracis
4.3.3.7 31000
-
 4 * 31000, calculated from sequence Bacillus anthracis
4.3.3.7 124000
-
 wild-type, sequence analysis Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Bacillus anthracis Q81WN7 Sterne strain
-
4.3.3.7 Bacillus anthracis Sterne Q81WN7 Sterne strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 by sonication, anion-exchange and hydrophobic interaction liquid chromatography Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate
-
 Bacillus anthracis dihydrodipicolinate + 2 H2O
-
 ?
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate
-
 Bacillus anthracis Sterne dihydrodipicolinate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.7 dimer 2 * 31000, calculated from sequence Bacillus anthracis
4.3.3.7 More the tetramer-dimer dissociation constant of the enzyme is 3fold tighter in the presence of pyruvate compared with the apo form Bacillus anthracis
4.3.3.7 tetramer 4 * 31000, calculated from sequence Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Bacillus anthracis
4.3.3.7 dihydrodipicolinate synthase
-
 Bacillus anthracis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.3.7 50 60 thermostability is significantly enhanced in the presence of the substrate pyruvate Bacillus anthracis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.3.7 1.7
-
 pyruvate mutant L170E/G191E, at 30°C Bacillus anthracis
4.3.3.7 92
-
 pyruvate wild-type, at 30°C Bacillus anthracis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.3.7 0.46
-
 pyruvate mutant L170E/G191E, at 30°C Bacillus anthracis
4.3.3.7 34
-
 L-aspartate 4-semialdehyde mutant L170E/G191E, at 30°C Bacillus anthracis
4.3.3.7 76.67
-
 pyruvate wild-type, at 30°C Bacillus anthracis
4.3.3.7 368
-
 L-aspartate 4-semialdehyde wild-type, at 30°C Bacillus anthracis