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Literature summary extracted from

  • Tan, M.; Zhu, B.; Zhou, X.L.; He, R.; Chen, X.; Eriani, G.; Wang, E.D.
    tRNA-dependent pre-transfer editing by prokaryotic leucyl-tRNA synthetase (2010), J. Biol. Chem., 285, 3235-3244.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.4 tRNALeu presence of tRNALeu robustly stimulates editing activity Escherichia coli
6.1.1.4 tRNALeu presence of tRNALeu robustly stimulates editing activity Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 T272R no change in aminoacylation activity, but the deacylation of Ile-tRNALeu is strongly impaired. Mutant still exhibits 45% of wild-type AMP formation Escherichia coli
6.1.1.4 T273R no change in aminoacylation activity, but the deacylation of Ile-tRNALeu is strongly impaired. Mutant still exhibits 70% of wild-type AMP formation Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole i.e. AN2690, 0.1 mM, 5fold decrease in aminoacylation activity Escherichia coli
6.1.1.4 additional information aminoacylation and editing reaction are resistant to inactivation by compound AN2690 Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Aquifex aeolicus
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6.1.1.4 Escherichia coli
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