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Literature summary extracted from

  • Wu, B.X.; Rajagopalan, V.; Roddy, P.L.; Clarke, C.J.; Hannun, Y.A.
    Identification and characterization of murine mitochondrial-associated neutral sphingomyelinase (MA-nSMase), the mammalian sphingomyelin phosphodiesterase 5 (2010), J. Biol. Chem., 285, 17993-18002.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 cardiolipin enzymatic activity is induced 23fold at 0.1 mM Mus musculus
3.1.4.12 additional information phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol have minimal effects on MA-nSMase activity Mus musculus
3.1.4.12 phosphatidylglycerol enzymatic activity is induced 8fold at 0.1 mM Mus musculus
3.1.4.12 phosphatidylserine enzymatic activity is induced 15fold at 0.1 mM Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in HEK-293 cells and MCF-7 cells Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 endoplasmic reticulum
-
 Mus musculus 5783
-
3.1.4.12 mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ absolute requirement for cations such as Mg2+ and Mn2+ Mus musculus
3.1.4.12 Mn2+ absolute requirement for cations such as Mg2+ and Mn2+ Mus musculus
3.1.4.12 additional information Ca2+, Co2+, Fe2+, or Zn2+ do not support MA-nSMase activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 50000
-
 SDS-PAGE Mus musculus
3.1.4.12 53870
-
 calculated from amino acid sequence Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Mus musculus D6MZJ6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 brain
-
 Mus musculus
-
3.1.4.12 epididymis
-
 Mus musculus
-
3.1.4.12 pancreas
-
 Mus musculus
-
3.1.4.12 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Mus musculus N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 MA-nSMase
-
 Mus musculus
3.1.4.12 mitochondrial-associated neutral sphingomyelinase
-
 Mus musculus
3.1.4.12 SMPD
-
 Mus musculus
3.1.4.12 sphingomyelin phosphodiesterase 5
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7.5
-
 the MA-nSMase has no acid SMase activity Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.12 Mus musculus calculated from amino acid sequence
-
 8.6