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Literature summary extracted from

  • Majtan, T.; Liu, L.; Carpenter, J.F.; Kraus, J.P.
    Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes (2010), J. Biol. Chem., 285, 15866-15873.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 S-adenosyl-L-methionine activity increases in the presence of Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.22 eight CBS mutants are expressed in Escherichia coli in the presence of chemical chaperones such as ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 A114V the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 E176K the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant does not respond to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 I435T the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 P422L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 P49L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 P78R the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 R125Q the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant does not respond to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens
4.2.1.22 S466L the presence of a chemical chaperone (ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide) in the medium during expression increases the mutant CBS activity in Escherichia coli crude extracts at least equal to wild-type, mutants show significant change in the level of active CBS tetramers in the presence of chaperones, mutant responds to a S-adenosyl-L-methionine stimulation or heating to 53°C with an increased activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-serine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.22 tetramer native gel Western blot Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 CBS
-
 Homo sapiens
4.2.1.22 cystathionine beta-synthase
-
 Homo sapiens