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Literature summary extracted from

  • Shaw, F.L.; Elliott, K.A.; Kinch, L.N.; Fuell, C.; Phillips, M.A.; Michael, A.J.
    Evolution and multifarious horizontal transfer of an alternative biosynthetic pathway for the alternative polyamine sym-homospermidine (2010), J. Biol. Chem., 285, 14711-14723.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Rhizobium leguminosarum
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Paramecium tetraurelia
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Blastochloris viridis
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Acinetobacter tartarogenes
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Ralstonia phage phiRSL1
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Opitutus terrae
2.5.1.44 expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Bradyrhizobium japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 0.05
-
 putrescine Vmax: 0.0014 mmol/min/mg Bradyrhizobium japonicum
2.5.1.44 0.26
-
 putrescine Vmax: 0.00132 mmol/min/mg Blastochloris viridis
2.5.1.44 0.28
-
 putrescine Vmax: 0.0088 mmol/min/mg Acinetobacter tartarogenes

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.43 Rhizobium leguminosarum
-
-
-
2.5.1.44 Acinetobacter tartarogenes
-
-
-
2.5.1.44 Blastochloris viridis
-
-
-
2.5.1.44 Bradyrhizobium japonicum Q89CN5
-
-
2.5.1.44 Opitutus terrae B1ZU21
-
-
2.5.1.44 Paramecium tetraurelia
-
-
-
2.5.1.44 Ralstonia phage phiRSL1 B2ZXX8
-
-
2.5.1.44 Rhizobium leguminosarum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.43 carboxynorspermidine + H2O + NADP+
-
 Rhizobium leguminosarum L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
 ?
1.5.1.43 carboxyspermidine + H2O + NADP+
-
 Rhizobium leguminosarum L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
 ?
2.5.1.44 2 putrescine
-
 Rhizobium leguminosarum sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Paramecium tetraurelia sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Blastochloris viridis sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Acinetobacter tartarogenes sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Ralstonia phage phiRSL1 sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Opitutus terrae sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine
-
 Bradyrhizobium japonicum sym-homospermidine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.43 CANSDH
-
 Rhizobium leguminosarum
1.5.1.43 carboxy(nor)spermidine dehydrogenase
-
 Rhizobium leguminosarum
1.5.1.43 carboxyspermidine dehydrogenase
-
 Rhizobium leguminosarum
2.5.1.44 homospermidine synthase orthologue
-
 Rhizobium leguminosarum
2.5.1.44 homospermidine synthase orthologue
-
 Paramecium tetraurelia
2.5.1.44 homospermidine synthase orthologue
-
 Blastochloris viridis
2.5.1.44 homospermidine synthase orthologue
-
 Acinetobacter tartarogenes
2.5.1.44 homospermidine synthase orthologue
-
 Ralstonia phage phiRSL1
2.5.1.44 homospermidine synthase orthologue
-
 Opitutus terrae
2.5.1.44 homospermidine synthase orthologue
-
 Bradyrhizobium japonicum
2.5.1.44 HSS orthologue
-
 Rhizobium leguminosarum
2.5.1.44 HSS orthologue
-
 Blastochloris viridis
2.5.1.44 HSS orthologue
-
 Acinetobacter tartarogenes
2.5.1.44 HSS orthologue
-
 Ralstonia phage phiRSL1
2.5.1.44 HSS orthologue
-
 Opitutus terrae
2.5.1.44 HSS orthologue
-
 Bradyrhizobium japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.44 28
-
 assay at Paramecium tetraurelia
2.5.1.44 28
-
 assay at Blastochloris viridis
2.5.1.44 28
-
 assay at Acinetobacter tartarogenes
2.5.1.44 28
-
 assay at Ralstonia phage phiRSL1
2.5.1.44 28
-
 assay at Opitutus terrae
2.5.1.44 28
-
 assay at Bradyrhizobium japonicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.44 1.18
-
 putrescine
-
 Bradyrhizobium japonicum
2.5.1.44 1.2
-
 putrescine
-
 Blastochloris viridis
2.5.1.44 8
-
 putrescine
-
 Acinetobacter tartarogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.44 8.4
-
 assay at Paramecium tetraurelia
2.5.1.44 8.4
-
 assay at Ralstonia phage phiRSL1
2.5.1.44 8.4
-
 assay at Opitutus terrae
2.5.1.44 8.4
-
 assay at, optimal pH Bradyrhizobium japonicum
2.5.1.44 8.7
-
 assay at, optimal pH Acinetobacter tartarogenes
2.5.1.44 8.8
-
 assay at, optimal pH Blastochloris viridis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+
-
 Rhizobium leguminosarum
2.5.1.44 NAD+
-
 Paramecium tetraurelia
2.5.1.44 NAD+
-
 Blastochloris viridis
2.5.1.44 NAD+
-
 Acinetobacter tartarogenes
2.5.1.44 NAD+
-
 Ralstonia phage phiRSL1
2.5.1.44 NAD+
-
 Opitutus terrae
2.5.1.44 NAD+
-
 Bradyrhizobium japonicum

General Information

EC Number General Information Comment Organism
2.5.1.44 malfunction sym-homospermidine is required for normal growth of the alpha-proteobacterium Rhizobium leguminosarum. Symhomospermidine can be replaced, for growth restoration, by the structural analogues spermidine and symnorspermidine, suggesting that the symmetrical or unsymmetrical form, and carbon backbone length are not critical for polyamine function in growth Rhizobium leguminosarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.44 4.6
-
 putrescine
-
 Blastochloris viridis
2.5.1.44 23.6
-
 putrescine
-
 Bradyrhizobium japonicum
2.5.1.44 28.6
-
 putrescine
-
 Acinetobacter tartarogenes