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Literature summary extracted from

  • Pacheco, B.; Malmstroem, A.; Maccarana, M.
    Two dermatan sulfate epimerases form iduronic acid domains in dermatan sulfate (2009), J. Biol. Chem., 284, 9788-9795.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.19 cloning and genomic structures of DS-epi1 and DS-epi2, transient functional overexpression of myc/His-tagged DS-epi1 and FLAG-tagged DS-epi2 in HEK-293 cell microsomes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.19 additional information siRNA gene silencing of DS-epi2 and DS-epi1 in lung HFL-1 fibroblasts Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.3.19 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.19 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.19 D-glucuronate Homo sapiens in dermatan sulfate, iduronic acid residues are either clustered together in blocks or alternating with glucuronic acid, forming hybrid structures L-iduronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.19 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.19 recombinant myc/His-tagged DS-epi1 and FLAG-tagged DS-epi2 partially in HEK-293 cell microsomes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.19 HFL-1 cell
-
 Homo sapiens
-
5.1.3.19 lung fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.19 D-glucuronate in dermatan sulfate, iduronic acid residues are either clustered together in blocks or alternating with glucuronic acid, forming hybrid structures Homo sapiens L-iduronate
-
 ?
5.1.3.19 D-glucuronate DS-epi2 has epimerase activity, which involves conversion of D-glucuronic acid to L-iduronic acid, EC 5.1.3.19, but no O-sulfotransferase activity, ovcerview Homo sapiens L-iduronate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.19 More domain structures of DS-epi1 and DS-epi2, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.1.3.19 dermatan sulfate epimerase
-
 Homo sapiens
5.1.3.19 DS-epi1
-
 Homo sapiens
5.1.3.19 DS-epi2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.19 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.19 6.8 7.5 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
5.1.3.19 malfunction DS-epi2 is genetically linked to bipolar disorder, which suggests that the dermatan sulfate domains generated by a defective enzyme may be involved in the etiology of the disease Homo sapiens
5.1.3.19 physiological function DS-epi2 and DS-epi1 are both involved in the biosynthesis of the iduronic acid blocks in fibroblasts and that DS-epi2 can also synthesize the hybrid structures Homo sapiens