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Literature summary extracted from

  • Salowe, S.P.; Wiltsie, J.; Hawkins, J.C.; Sonatore, L.M.
    The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase (2009), J. Biol. Chem., 284, 9656-9662.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.5 into the vector pET3D-FLAG for expression in Escherichia coli BL21DE3 cells Bacillus subtilis
6.3.4.19
-
Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.19 (5R)-5-hydroxy-L-lysine
-
Escherichia coli
6.3.4.19 4,5-trans-dehydro-L-lysine
-
Escherichia coli
6.3.4.19 8-azaadenosine 5’-triphosphate
-
Escherichia coli
6.3.4.19 adenosine 5’-O-(1-thiotriphosphate)
-
Escherichia coli
6.3.4.19 alpha,beta-methyleneadenosine 5'-triphosphate
-
Escherichia coli
6.3.4.19 aminoethylcysteine
-
Escherichia coli
6.3.4.19 aminoethylserine
-
Escherichia coli
6.3.4.19 benzimidazoleriboside 5’-triphosphate
-
Escherichia coli
6.3.4.19 beta,gamma-methyleneadenosine 5'-triphosphate
-
Escherichia coli
6.3.4.19 cadaverine
-
Escherichia coli
6.3.4.19 ethanolamine
-
Escherichia coli
6.3.4.19 histamine
-
Escherichia coli
6.3.4.19 L-lysinamide
-
Escherichia coli
6.3.4.19 L-ornithine
-
Escherichia coli
6.3.4.19 mutated tRNAIle2 C34G-tRNAILe2 Escherichia coli
6.3.4.19 N-acetylethylenediamine
-
Escherichia coli
6.3.4.19 N-alpha-methyl-L-lysine
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.19 0.0016
-
ATP pH 8.5, 21°C Escherichia coli
6.3.4.19 0.0018
-
7-deazaadenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli
6.3.4.19 0.01
-
8-azidoadenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli
6.3.4.19 0.0217
-
N6-methyladenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle Bacillus subtilis bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
6.1.1.5 ATP + L-isoleucine + tRNAIle Bacillus subtilis 168 bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + ATP Escherichia coli bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Bacillus subtilis Q45477
-
-
6.1.1.5 Bacillus subtilis 168 Q45477
-
-
6.3.4.19 Escherichia coli P52097
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.5 using M2-FLAG resin Bacillus subtilis
6.3.4.19
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS Bacillus subtilis AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
6.1.1.5 ATP + L-isoleucine + tRNAIle bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS Bacillus subtilis 168 AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
6.3.4.19 additional information no substrate: N1-methyladenosine-5'-triphosphate, 2-aminoadenosine-5'-triphosphate, 2-amino-6-chloropurineriboside-5'-triphosphate, and 6-chloropurineriboside-5'-triphosphate Escherichia coli ?
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + 7-deazaadenosine 5'-triphosphate Vmax/Km is 8.6% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + 7-deazaadenosine 5'-phosphate + diphosphate
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + 8-azidoadenosine 5'-triphosphate Vmax/Km is 2.3% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + 8-azidoadenosine 5'-phosphate + diphosphate
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + ATP
-
Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + ATP bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?
6.3.4.19 [tRNAIle2]-cytidine34 + L-lysine + N6-methyladenosine-5'-triphosphate Vmax/Km is 4.7% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + N6-methyladenosine-5'-phosphate + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.5 IleRS
-
Bacillus subtilis
6.1.1.5 Isoleucyl-tRNA synthetase
-
Bacillus subtilis
6.3.4.19 tRNAIle-lysidine synthetase
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.5 22
-
activity assay at room temperature Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.5 7.3
-
activity assay Bacillus subtilis
6.3.4.19 8.5 9.5
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.5 ATP
-
Bacillus subtilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.4.19 0.000016
-
pH 8.5, 21°C Escherichia coli adenosine-5’-O-(1-thiotriphosphate)
6.3.4.19 0.000035
-
pH 8.5, 21°C Escherichia coli alpha,beta-methylene-ATP
6.3.4.19 0.000045
-
pH 8.5, 21°C Escherichia coli aminoethylcysteine
6.3.4.19 0.000082
-
pH 8.5, 21°C Escherichia coli beta,gamma-methyleneadenosine 5'-triphosphate
6.3.4.19 0.000085
-
pH 8.5, 21°C, C34G-tRNAILe2 Escherichia coli mutated tRNAIle2
6.3.4.19 0.0004
-
pH 8.5, 21°C Escherichia coli aminoethylserine
6.3.4.19 0.0025
-
pH 8.5, 21°C Escherichia coli 4,5-trans-dehydrolysine
6.3.4.19 0.0068
-
pH 8.5, 21°C Escherichia coli N-acetylethylenediamine
6.3.4.19 0.0176
-
pH 8.5, 21°C Escherichia coli (5R)-5-hydroxylysine
6.3.4.19 0.105
-
pH 8.5, 21°C Escherichia coli benzimidazoleriboside-5’-triphosphate
6.3.4.19 0.255
-
pH 8.5, 21°C Escherichia coli 8-azaadenosine-5’-triphosphate
6.3.4.19 0.33
-
pH 8.5, 21°C Escherichia coli histamine
6.3.4.19 0.62
-
pH 8.5, 21°C Escherichia coli lysinamide
6.3.4.19 0.7
-
pH 8.5, 21°C Escherichia coli ornithine
6.3.4.19 0.91
-
pH 8.5, 21°C Escherichia coli cadaverine
6.3.4.19 1.3
-
pH 8.5, 21°C Escherichia coli N-alpha-methyl-lysine
6.3.4.19 1.77
-
pH 8.5, 21°C Escherichia coli ethanolamine

General Information

EC Number General Information Comment Organism
6.3.4.19 physiological function bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase Escherichia coli