EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.5 | into the vector pET3D-FLAG for expression in Escherichia coli BL21DE3 cells | Bacillus subtilis |
6.3.4.19 | - |
Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.19 | (5R)-5-hydroxy-L-lysine | - |
Escherichia coli | |
6.3.4.19 | 4,5-trans-dehydro-L-lysine | - |
Escherichia coli | |
6.3.4.19 | 8-azaadenosine 5-triphosphate | - |
Escherichia coli | |
6.3.4.19 | adenosine 5-O-(1-thiotriphosphate) | - |
Escherichia coli | |
6.3.4.19 | alpha,beta-methyleneadenosine 5'-triphosphate | - |
Escherichia coli | |
6.3.4.19 | aminoethylcysteine | - |
Escherichia coli | |
6.3.4.19 | aminoethylserine | - |
Escherichia coli | |
6.3.4.19 | benzimidazoleriboside 5-triphosphate | - |
Escherichia coli | |
6.3.4.19 | beta,gamma-methyleneadenosine 5'-triphosphate | - |
Escherichia coli | |
6.3.4.19 | cadaverine | - |
Escherichia coli | |
6.3.4.19 | ethanolamine | - |
Escherichia coli | |
6.3.4.19 | histamine | - |
Escherichia coli | |
6.3.4.19 | L-lysinamide | - |
Escherichia coli | |
6.3.4.19 | L-ornithine | - |
Escherichia coli | |
6.3.4.19 | mutated tRNAIle2 | C34G-tRNAILe2 | Escherichia coli | |
6.3.4.19 | N-acetylethylenediamine | - |
Escherichia coli | |
6.3.4.19 | N-alpha-methyl-L-lysine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.19 | 0.0016 | - |
ATP | pH 8.5, 21°C | Escherichia coli | |
6.3.4.19 | 0.0018 | - |
7-deazaadenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli | |
6.3.4.19 | 0.01 | - |
8-azidoadenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli | |
6.3.4.19 | 0.0217 | - |
N6-methyladenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.5 | ATP + L-isoleucine + tRNAIle | Bacillus subtilis | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | Bacillus subtilis 168 | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + ATP | Escherichia coli | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.5 | Bacillus subtilis | Q45477 | - |
- |
6.1.1.5 | Bacillus subtilis 168 | Q45477 | - |
- |
6.3.4.19 | Escherichia coli | P52097 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.5 | using M2-FLAG resin | Bacillus subtilis |
6.3.4.19 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.5 | ATP + L-isoleucine + tRNAIle | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS | Bacillus subtilis | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine, the lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by IleRS | Bacillus subtilis 168 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.3.4.19 | additional information | no substrate: N1-methyladenosine-5'-triphosphate, 2-aminoadenosine-5'-triphosphate, 2-amino-6-chloropurineriboside-5'-triphosphate, and 6-chloropurineriboside-5'-triphosphate | Escherichia coli | ? | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + 7-deazaadenosine 5'-triphosphate | Vmax/Km is 8.6% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + 7-deazaadenosine 5'-phosphate + diphosphate | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + 8-azidoadenosine 5'-triphosphate | Vmax/Km is 2.3% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + 8-azidoadenosine 5'-phosphate + diphosphate | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + ATP | - |
Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + ATP | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
6.3.4.19 | [tRNAIle2]-cytidine34 + L-lysine + N6-methyladenosine-5'-triphosphate | Vmax/Km is 4.7% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + N6-methyladenosine-5'-phosphate + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.5 | IleRS | - |
Bacillus subtilis |
6.1.1.5 | Isoleucyl-tRNA synthetase | - |
Bacillus subtilis |
6.3.4.19 | tRNAIle-lysidine synthetase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.5 | 22 | - |
activity assay at room temperature | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.5 | 7.3 | - |
activity assay | Bacillus subtilis |
6.3.4.19 | 8.5 | 9.5 | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.5 | ATP | - |
Bacillus subtilis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
6.3.4.19 | 0.000016 | - |
pH 8.5, 21°C | Escherichia coli | adenosine-5-O-(1-thiotriphosphate) | |
6.3.4.19 | 0.000035 | - |
pH 8.5, 21°C | Escherichia coli | alpha,beta-methylene-ATP | |
6.3.4.19 | 0.000045 | - |
pH 8.5, 21°C | Escherichia coli | aminoethylcysteine | |
6.3.4.19 | 0.000082 | - |
pH 8.5, 21°C | Escherichia coli | beta,gamma-methyleneadenosine 5'-triphosphate | |
6.3.4.19 | 0.000085 | - |
pH 8.5, 21°C, C34G-tRNAILe2 | Escherichia coli | mutated tRNAIle2 | |
6.3.4.19 | 0.0004 | - |
pH 8.5, 21°C | Escherichia coli | aminoethylserine | |
6.3.4.19 | 0.0025 | - |
pH 8.5, 21°C | Escherichia coli | 4,5-trans-dehydrolysine | |
6.3.4.19 | 0.0068 | - |
pH 8.5, 21°C | Escherichia coli | N-acetylethylenediamine | |
6.3.4.19 | 0.0176 | - |
pH 8.5, 21°C | Escherichia coli | (5R)-5-hydroxylysine | |
6.3.4.19 | 0.105 | - |
pH 8.5, 21°C | Escherichia coli | benzimidazoleriboside-5-triphosphate | |
6.3.4.19 | 0.255 | - |
pH 8.5, 21°C | Escherichia coli | 8-azaadenosine-5-triphosphate | |
6.3.4.19 | 0.33 | - |
pH 8.5, 21°C | Escherichia coli | histamine | |
6.3.4.19 | 0.62 | - |
pH 8.5, 21°C | Escherichia coli | lysinamide | |
6.3.4.19 | 0.7 | - |
pH 8.5, 21°C | Escherichia coli | ornithine | |
6.3.4.19 | 0.91 | - |
pH 8.5, 21°C | Escherichia coli | cadaverine | |
6.3.4.19 | 1.3 | - |
pH 8.5, 21°C | Escherichia coli | N-alpha-methyl-lysine | |
6.3.4.19 | 1.77 | - |
pH 8.5, 21°C | Escherichia coli | ethanolamine |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.3.4.19 | physiological function | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | Escherichia coli |