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Literature summary extracted from

  • Ruiz-Duenas, F.J.; Pogni, R.; Morales, M.; Giansanti, S.; Mate, M.J.; Romero, A.; Martinez, M.J.; Basosi, R.; Martinez, A.T.
    Protein radicals in fungal versatile peroxidase: catalytic tryptophan radical in both compound I and compound II and studies on W164Y, W164H, and W164S variants (2009), J. Biol. Chem., 284, 7986-7994.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expression in Escherichia coli W3110 Pleurotus eryngii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.16 mutant enzyme W164Y, sitting-drop vapor diffusion method, resolution 1.94 A Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 R257A/A260F site-directed mutagenesis Pleurotus eryngii
1.11.1.16 W164H site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
1.11.1.16 W164S site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
1.11.1.16 W164Y site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
1.11.1.16 W164Y/R257A/A260F site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.0034
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
1.11.1.16 0.0049
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 0.076
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii
1.11.1.16 0.078
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
1.11.1.16 0.11
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
1.11.1.16 0.133
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii
1.11.1.16 0.15
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 4.13
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
1.11.1.16 13.5
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.16 resource-Q chromatography Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 Mn2+ + H2O2
-
 Pleurotus eryngii ?
-
 ?
1.11.1.16 Reactive Black 5 + H2O2
-
 Pleurotus eryngii ?
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.16 versatile peroxidase
-
 Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 5.5
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
1.11.1.16 9.1
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 9.5
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
1.11.1.16 17.9
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 164
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
1.11.1.16 207
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 207
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
1.11.1.16 216
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii
1.11.1.16 275
-
 Mn2+ native recombinant enzyme Pleurotus eryngii
1.11.1.16 328
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.16 1.3
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 2.3
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
1.11.1.16 1380
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 1520
-
 Mn2+ native recombinant enzyme Pleurotus eryngii
1.11.1.16 1610
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
1.11.1.16 1900
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
1.11.1.16 1900
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
1.11.1.16 2110
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
1.11.1.16 2470
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii
1.11.1.16 2850
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii