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Literature summary extracted from

  • Hidaka, M.; Nishimoto, M.; Kitaoka, M.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold (2009), J. Biol. Chem., 284, 7273-7283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.211 the non-labeled enzyme is expressed in Escherichia coli BL21(DE3) cells, the selenomethionine-labeled enzyme is expressed in Escherichia coli strain B834 (DE3) Bifidobacterium longum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.211 sitting drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 0.2 M Mg(NO3)2, and 15% (v/v) polyethylene glycol 4000 Bifidobacterium longum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.211 D313N mutant with undetectable activity Bifidobacterium longum
2.4.1.211 F364N mutant shows severely impaired activity Bifidobacterium longum
2.4.1.211 N166A mutant shows severely impaired activity Bifidobacterium longum
2.4.1.211 R210E the mutant shows no detectable activity Bifidobacterium longum
2.4.1.211 R32E the mutant shows no detectable activity Bifidobacterium longum
2.4.1.211 R358E the mutant shows no detectable activity Bifidobacterium longum
2.4.1.211 Y362F the mutation results in about 1000fold reduction of the catalytic efficiency Bifidobacterium longum
2.4.1.211 Y362N the mutation results in the complete loss of the activity Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.211 1.72
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 17
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 19
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 24
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.211 Bifidobacterium longum E8MF13
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.211
-
Bifidobacterium longum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
-
Bifidobacterium longum alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Bifidobacterium longum alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.211 Gal-HexNAcP
-
Bifidobacterium longum
2.4.1.211 galacto-N-biose/lacto-N-biose I phosphorylase
-
Bifidobacterium longum
2.4.1.211 GLNBP
-
Bifidobacterium longum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.211 0.09
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 0.3
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 4.83
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 10.7
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum

General Information

EC Number General Information Comment Organism
2.4.1.211 physiological function key enzyme for intestinal growth, phosphorolyses galacto-N-biose and lacto-N-biose I with anomeric inversion Bifidobacterium longum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.211 0.005
-
alpha-D-galactose 1-phosphate mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 0.012
-
alpha-D-galactose 1-phosphate mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 0.26
-
alpha-D-galactose 1-phosphate mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum
2.4.1.211 6.21
-
alpha-D-galactose 1-phosphate wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate Bifidobacterium longum