EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.180 | gene fabH, overexpression of His-tagged wild-type and mutant FabHs in Mycobacterium bovis strain BCG | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.180 | mapping of the Thr45 phosphorylation site on the mtFabH crystal structure, overview | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.180 | T45A | the mutant exhibits slightly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein | Mycobacterium tuberculosis |
2.3.1.180 | T45D | the mutant exhibits markedly decreased transacylation, malonyl-AcpM decarboxylation, and condensing activities compared to the wild-type protein | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis H37Rv | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | additional information | Mycobacterium tuberculosis | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | ? | - |
? | |
2.3.1.180 | additional information | Mycobacterium tuberculosis H37Rv | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.180 | Mycobacterium tuberculosis | P9WNG3 | gene fabH | - |
2.3.1.180 | Mycobacterium tuberculosis H37Rv | P9WNG3 | gene fabH | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.3.1.180 | phosphoprotein | FabH is efficiently phosphorylated in vitro exclusively on Thr45 by several mycobacterial Ser/Thr protein kinases, particularly by PknF and PknA, as well as in vivo in mycobacteria, mass spectrometric analysis, overview. Mapping of the Thr45 phosphorylation site on the FabH crystal structure | Mycobacterium tuberculosis |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.180 | recombinant His-tagged wild-type and mutant FabHs from Mycobacterium bovis strain BCG by nickel affinity chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | Mycobacterium tuberculosis | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis H37Rv | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP | Mycobacterium tuberculosis H37Rv | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | additional information | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | Mycobacterium tuberculosis | ? | - |
? | |
2.3.1.180 | additional information | FabH is regulated by Ser/Thr protein kinase-dependent phosphorylation at Thr45, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.180 | beta-ketoacyl-ACP synthase III | - |
Mycobacterium tuberculosis |
2.3.1.180 | beta-ketoacyl-acyl carrier protein synthase III | - |
Mycobacterium tuberculosis |
2.3.1.180 | FabH | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 37 | - |
condensation and decarboxylation assays at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 7 | - |
condensation assay at | Mycobacterium tuberculosis |
2.3.1.180 | 7.5 | - |
decarboxylation assay at | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.180 | physiological function | FabH links FAS-I and FAS-II catalyzing the condensation of FAS-I-derived acyl-CoAs with malonyl-ACP. FabH represents a potential regulatory key point of the mycolic acid pathway | Mycobacterium tuberculosis |