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Literature summary extracted from

  • Min, L.; Jin, Z.; Caldovic, L.; Morizono, H.; Allewell, N.M.; Tuchman, M.; Shi, D.
    Mechanism of allosteric inhibition of N-acetyl-L-glutamate synthase by L-arginine (2009), J. Biol. Chem., 284, 4873-4880.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.1 expression in Escherichia coli BL21 Neisseria gonorrhoeae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.1 hanging drop method Neisseria gonorrhoeae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.1 L-arginine
-
 Neisseria gonorrhoeae
2.7.2.8 L-arginine
-
 Arabidopsis thaliana
2.7.2.8 L-arginine
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.1 0.1
-
 acetyl-CoA
-
 Neisseria gonorrhoeae
2.3.1.1 4.5
-
 L-glutamate
-
 Neisseria gonorrhoeae
2.3.1.1 6.6
-
 L-glutamate in presence of 0.1 mM L-arginine Neisseria gonorrhoeae
2.3.1.1 27.5
-
 L-glutamate in presence of 1 mM L-arginine Neisseria gonorrhoeae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.1 330000
-
 gel filtration Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate Arabidopsis thaliana
-
 ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate Pseudomonas aeruginosa
-
 ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Neisseria gonorrhoeae Q5FAK7
-
-
2.7.2.8 Arabidopsis thaliana
-
-
-
2.7.2.8 Pseudomonas aeruginosa Q9HTN2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 nickel affinity and DEAE columns Neisseria gonorrhoeae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 4.4
-
 residual activity at inhibition by L-arginine Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate assay at pH 8.5, 30°C, inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae CoA + N-acetyl-L-glutamate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Arabidopsis thaliana ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.1 hexamer inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.1 N-acetyl-L-glutamate synthase inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae
2.3.1.1 NAGS
-
 Neisseria gonorrhoeae
2.3.1.1 ngNAGS
-
 Neisseria gonorrhoeae
2.7.2.8 N-acetyl-L-glutamate kinase
-
 Arabidopsis thaliana
2.7.2.8 N-acetyl-L-glutamate kinase
-
 Pseudomonas aeruginosa
2.7.2.8 NagK
-
 Arabidopsis thaliana
2.7.2.8 NagK
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.1 30
-
 assay at Neisseria gonorrhoeae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.1 8.5
-
 assay at Neisseria gonorrhoeae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.8 ATP
-
 Arabidopsis thaliana
2.7.2.8 ATP
-
 Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.1 0.19
-
-
 Neisseria gonorrhoeae L-arginine

General Information

EC Number General Information Comment Organism
2.3.1.1 physiological function first step in L-arginine biosynthesis in plants and micro organsims, inactive T-state with L-arginine bound and active R-state complexed with CoA and L-glutamate Neisseria gonorrhoeae