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Literature summary extracted from

  • Mollicone, R.; Moore, S.E.; Bovin, N.; Garcia-Rosasco, M.; Candelier, J.J.; Martinez-Duncker, I.; Oriol, R.
    Activity, splice variants, conserved peptide motifs, and phylogeny of two new alpha1,3-fucosyltransferase families (FUT10 and FUT11) (2009), J. Biol. Chem., 284, 4723-4738.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.65 transient expression of cDNA splice variants FUT10-391, FUT10-419, and FUT10-479, and GFP-tagged-FUT10 cDNA constructs from vector PCR3.1 in COS-7 Cells Homo sapiens
2.4.1.65 transient expression of cDNA splice variants FUT11-476 and FUT11-492 from vector PCR3.1 in COS-7 Cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.65 0.013
-
 GDP-L-fucose FUT10-391 Homo sapiens
2.4.1.65 0.015
-
 GDP-L-fucose FUT10-479 Homo sapiens
2.4.1.65 0.15
-
 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin FUT10-479 Homo sapiens
2.4.1.65 0.154
-
 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin FUT10-391 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.65 endoplasmic reticulum FUT10-419 and FUT10-479, FUT10-479 has the same trans-membrane domain and NH2-terminal endoplasmic reticulum membrane retention signal found in the FUT10-419 splice variant plus a new endoplasmic reticulum membrane retention signal in the COOH terminus Homo sapiens 5783
-
2.4.1.65 endoplasmic reticulum FUT11-476 has no endoplasmic reticulum membrane retention signal, but the FUT11-492 variant has a KKXX-like motif in its COOH terminus that can retain it in the endoplasmic reticulum Homo sapiens 5783
-
2.4.1.65 Golgi apparatus FUT10-391 is a soluble protein without a transmembrane domain or endoplasmic reticulum retention signal that transiently localizes to the Golgi and then is routed to the lysosome Homo sapiens 5794
-
2.4.1.65 membrane FUT10-419 and FUT10-479 have a type II trans-membrane topology. FUT10-479 has, in addition, a COOH-endoplasmic reticulum membrane retention signal Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.65 additional information FUT10 functions well without MnCl2 Homo sapiens
2.4.1.65 additional information FUT11 functions well without MnCl2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens Q495W5
-
-
2.4.1.65 Homo sapiens Q6P4F1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.65 brain FUT10-479, adult tissue with low level Homo sapiens
-
2.4.1.65 colon adult tissue with high level Homo sapiens
-
2.4.1.65 embryo FUT10-391 and FUT10-419 Homo sapiens
-
2.4.1.65 gall bladder adult tissue with high level Homo sapiens
-
2.4.1.65 ileum adult tissue with weak level Homo sapiens
-
2.4.1.65 jejunum adult tissue with high level Homo sapiens
-
2.4.1.65 kidney fetal tissue with weak level, adult tissue with moderate level Homo sapiens
-
2.4.1.65 liver fetal tissue, intensity similar to in the embryos Homo sapiens
-
2.4.1.65 lung fetal and adult tissue with weak level Homo sapiens
-
2.4.1.65 additional information adult spleen, heart, muscle, liver, and pancreas do not express any of the FUT10 transcripts Homo sapiens
-
2.4.1.65 muscle fetal tissue with weak level Homo sapiens
-
2.4.1.65 placenta adult tissue with weak level Homo sapiens
-
2.4.1.65 rectum adult tissue with high level Homo sapiens
-
2.4.1.65 skin fetal tissue, intensity similar to in the embryos Homo sapiens
-
2.4.1.65 small intestine fetal tissue, intensity similar to in the embryos Homo sapiens
-
2.4.1.65 stomach adult tissue with high level Homo sapiens
-
2.4.1.65 uterus adult tissue with moderate level Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
 Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose FUT10 activity toward the acceptor 0989-BM is increased by about 50% compared with those observed using BGA-biotin Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin + GDP-L-fucose
-
 Homo sapiens GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)AspNHCO-biotin + GDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.65 alpha1,3-fucosyltransferase
-
 Homo sapiens
2.4.1.65 FUT10
-
 Homo sapiens
2.4.1.65 FUT11
-
 Homo sapiens