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Literature summary extracted from

  • Kuhn, M.L.; Falaschetti, C.A.; Ballicora, M.A.
    Ostreococcus tauri ADP-glucose pyrophosphorylase reveals alternative paths for the evolution of subunit roles (2009), J. Biol. Chem., 284, 34092-34102.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate
-
 Ostreococcus tauri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 the S and L subunit genes are inserted into the pSC-B vector using the StrataClone Blunt PCR cloning kit, S ann L subunits are subcloned into pMAB5 and pMAB6 expression vectors, respectively, for expression in Escherichia coli AC7OR1-504 cells Ostreococcus tauri

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 OtaL_D171A mutant subunit L Ostreococcus tauri
2.7.7.27 OtaL_K224R mutant subunit L Ostreococcus tauri
2.7.7.27 OtaS_D148A mutant subunit S Ostreococcus tauri
2.7.7.27 OtaS_D148A/K201R double mutant subunit S Ostreococcus tauri
2.7.7.27 OtaS_K201R mutant subunit S Ostreococcus tauri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate
-
 Ostreococcus tauri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+
-
 Ostreococcus tauri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 50000
-
 S subunit, determined by SDS-PAGE Ostreococcus tauri
2.7.7.27 52000
-
 L subunit, determined by SDS-PAGE Ostreococcus tauri
2.7.7.27 204000
-
 heterotetramer, S2L2, calculated Ostreococcus tauri
2.7.7.27 222000
-
 heterotetramer, S2L2, determined by gel filtration Ostreococcus tauri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Ostreococcus tauri
-
 diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Ostreococcus tauri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 using a DEAE-Sepharose Fast Flow weak anion exchange and a Source 15Q 4.6/100 PE strong anion exchange column, and a Resource PHE 1-ml HIC hydrophobic column Ostreococcus tauri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Ostreococcus tauri diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer the enzyme includes two distinct S and L homologous subunits, S2L2 or alpha2beta2 Ostreococcus tauri

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 ADP-Glc PPase
-
 Ostreococcus tauri
2.7.7.27 ADP-glucose pyrophosphorylase
-
 Ostreococcus tauri
2.7.7.27 OtaL Ostreococcus tauri ADP-glucose pyrophosphorylase subunit L Ostreococcus tauri
2.7.7.27 OtaS Ostreococcus tauri ADP-glucose pyrophosphorylase subunit S Ostreococcus tauri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.27 37
-
 activity assay Ostreococcus tauri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 8
-
 activity assay Ostreococcus tauri

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.27 ATP
-
 Ostreococcus tauri

General Information

EC Number General Information Comment Organism
2.7.7.27 physiological function ADP-glucose pyrophosphorylase controls starch synthesis in plants Ostreococcus tauri