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Literature summary extracted from

  • Westrop, G.D.; Georg, I.; Coombs, G.H.
    The mercaptopyruvate sulfurtransferase of Trichomonas vaginalis links cysteine catabolism to the production of thioredoxin persulfide (2009), J. Biol. Chem., 284, 33485-33494.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 0.0028
-
 thioredoxin pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 0.053
-
 3-Mercaptopyruvate pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 8.2
-
 cysteine pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 19
-
 2-mercaptoethanol pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 266
-
 thiosulfate pH 8.4, 30°C Trichomonas vaginalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.2 32700
-
 x * 32700, calculated, x * 33000, SDS-PAGE Trichomonas vaginalis
2.8.1.2 33000
-
 x * 32700, calculated, x * 33000, SDS-PAGE Trichomonas vaginalis

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Trichomonas vaginalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + 2-mercaptoethanol
-
 Trichomonas vaginalis pyruvate + ?
-
 ?
2.8.1.2 cysteine + 2-mercaptoethanol
-
 Trichomonas vaginalis ? + ?
-
 ?
2.8.1.2 additional information has high activity with 3-MP as a sulfur donor and can use several thiol compounds as sulfur acceptor substrates Trichomonas vaginalis ?
-
 ?
2.8.1.2 thioredoxin + 2-mercaptoethanol
-
 Trichomonas vaginalis thioredoxin persulfide + ?
-
 ?
2.8.1.2 thiosulfate + 2-mercaptoethanol
-
 Trichomonas vaginalis sulfate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.2 ? x * 32700, calculated, x * 33000, SDS-PAGE Trichomonas vaginalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.2 11.3
-
 thiosulfate pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 172
-
 thioredoxin pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 2240
-
 cysteine pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 2260
-
 3-Mercaptopyruvate pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 5310
-
 2-mercaptoethanol pH 8.4, 30°C Trichomonas vaginalis

General Information

EC Number General Information Comment Organism
2.8.1.2 physiological function changes in MST activity in response to variation in the supply of exogenous cysteine are suggestive of a role for the mercaptopyruvate pathway in the removal of excess intracellular cysteine, redox homeostasis, and antioxidant defense Trichomonas vaginalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.1.2 0.0427
-
 thiosulfate pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 270
-
 cysteine pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 390
-
 2-mercaptoethanol pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 35000
-
 3-Mercaptopyruvate pH 8.4, 30°C Trichomonas vaginalis
2.8.1.2 62000
-
 thioredoxin pH 8.4, 30°C Trichomonas vaginalis