Literature summary extracted from
Pena, M.M.; Melo, S.P.; Xing, Y.Y.; White, K.; Barbour, K.W.; Berger, F.G.
The intrinsically disordered N-terminal domain of thymidylate synthase targets the enzyme to the ubiquitin-independent proteasomal degradation pathway (2009), J. Biol. Chem., 284, 31597-31607.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.45 |
expressed in RJK88.13 cells and in HEp2/500 cells |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.1.45 |
G5L/S6A |
the mutant has a relatively short half-life of about 1-2 h |
Mus musculus |
2.1.1.45 |
P2A |
the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h |
Mus musculus |
2.1.1.45 |
P2D |
the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h |
Mus musculus |
2.1.1.45 |
P2G |
the mutant is unstable relative to wild type enzyme having half-life of 6-8 h similar to wild type |
Mus musculus |
2.1.1.45 |
P2R |
the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h |
Mus musculus |
2.1.1.45 |
P2V |
the mutant is unstable relative to wild type enzyme having half-life of 1-2 h with half-life of more than 36-48 h |
Mus musculus |
2.1.1.45 |
P2W |
the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h |
Mus musculus |
2.1.1.45 |
P2Y |
the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h |
Mus musculus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.45 |
Mus musculus |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.1.1.45 |
acetylation |
Nalpha-acetylation inhibits thymidylate degradation, Pro-2 protects the N terminus against Nalpha-acetylation |
Mus musculus |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.45 |
HiTrap Blue-Sepharose column chromatography and HiTrap Q column chromatography |
Mus musculus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.45 |
5,10-methylenetetrahydrofolate + dUMP |
- |
Mus musculus |
dihydrofolate + dTMP |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.45 |
N5,N10-methylenetetrahydrofolate |
- |
Mus musculus |
|