BRENDA - Enzyme Database

Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol

Lung, M.; Shulga, Y.V.; Ivanova, P.T.; Myers, D.S.; Milne, S.B.; Brown, H.A.; Topham, M.K.; Epand, R.M.; J. Biol. Chem. 284, 31062-31073 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.107
expressed in COS-7 cells and in baculovirus-infected Sf21 insect cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
1,2-diarachidonoyl phosphatidic acid
about 55% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-arachidoyl-2-arachidonoyl phosphatidic acid
about 70% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-palmitoyl-2-arachidonoyl phosphatidic acid
about 50% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-stearoyl-2-arachidonoyl phosphatidic acid
about 48% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-stearoyl-2-oleoyl phosphatidic acid
about 70% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
phosphatidic acid
competitive inhibition
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Homo sapiens
P52429
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
isoform DGKepsilon shows about 7% activity with 0.38 mol% 1,2-dioleoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-arachidoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows about 70% activity with 0.38 mol% 1-arachidoyl-2-arachidonoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.107
ATP + 1-palmitoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows about 90% activity with 0.38 mol% 1-palmitoyl-2-arachidonoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows 100% activity with 0.38 mol% 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows substrate specificity for sn-2 arachidonoyl-diacylglycerol
704564
Homo sapiens
ADP + 2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.107
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
expressed in COS-7 cells and in baculovirus-infected Sf21 insect cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.107
ATP
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
1,2-diarachidonoyl phosphatidic acid
about 55% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-arachidoyl-2-arachidonoyl phosphatidic acid
about 70% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-palmitoyl-2-arachidonoyl phosphatidic acid
about 50% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-stearoyl-2-arachidonoyl phosphatidic acid
about 48% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
1-stearoyl-2-oleoyl phosphatidic acid
about 70% relative activity in the presence of 1.5 mol%
Homo sapiens
2.7.1.107
phosphatidic acid
competitive inhibition
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
isoform DGKepsilon shows about 7% activity with 0.38 mol% 1,2-dioleoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-arachidoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows about 70% activity with 0.38 mol% 1-arachidoyl-2-arachidonoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.107
ATP + 1-palmitoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows about 90% activity with 0.38 mol% 1-palmitoyl-2-arachidonoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows 100% activity with 0.38 mol% 1-stearoyl-2-arachidonoyl-sn-glycerol
704564
Homo sapiens
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 2-arachidonoyl-sn-glycerol
isoform DGKepsilon shows substrate specificity for sn-2 arachidonoyl-diacylglycerol
704564
Homo sapiens
ADP + 2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?