BRENDA - Enzyme Database

A novel biosynthetic pathway of archaetidyl-myo-inositol via archaetidyl-myo-inositol phosphate from CDP-archaeol and D-glucose 6-phosphate in methanoarchaeon Methanothermobacter thermautotrophicus cells

Morii, H.; Kiyonari, S.; Ishino, Y.; Koga, Y.; J. Biol. Chem. 284, 30766-30774 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.39
Triton X-100
0.5%, stimulates
Methanothermobacter thermautotrophicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.8.39
expression in Escherichia coli
Methanothermobacter thermautotrophicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.B14
phosphate
8–20 mM phosphate buffer inhibits
Methanothermobacter thermautotrophicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.39
membrane
-
Methanothermobacter thermautotrophicus
16020
-
2.7.8.39
membrane
-
Pyrococcus furiosus
16020
-
3.1.3.B14
membrane
-
Methanothermobacter thermautotrophicus
16020
-
5.5.1.4
membrane
-
Methanothermobacter thermautotrophicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.39
Mg2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
2.7.8.39
Mn2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.39
20764
-
x * 20764, calculated from sequence
Methanothermobacter thermautotrophicus
2.7.8.39
20900
-
x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea
1-archaetidyl-1D-myo-inositol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.39
Methanothermobacter thermautotrophicus
O27726
-
-
2.7.8.39
Pyrococcus furiosus
-
-
-
3.1.3.B14
Methanothermobacter thermautotrophicus
-
-
-
5.5.1.4
Methanothermobacter thermautotrophicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.5.1.4
partially purified by ammonium sulfate fractionation (50-70%)
Methanothermobacter thermautotrophicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.39
0.02
-
pH 8.0, 60°C, crude enzyme
Methanothermobacter thermautotrophicus
5.5.1.4
0.0003
-
crude enzyme, in 50mM Bicine buffer (pH 8.5), at 60°C
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
-
704559
Methanothermobacter thermautotrophicus
1-archaetidyl-1D-myo-inositol + phosphate
-
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea
704559
Methanothermobacter thermautotrophicus
1-archaetidyl-1D-myo-inositol + phosphate
-
-
-
?
5.5.1.4
D-glucose 6-phosphate
-
704559
Methanothermobacter thermautotrophicus
1L-myo-inositol 1-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.39
?
x * 20764, calculated from sequence; x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.39
60
-
assay at
Methanothermobacter thermautotrophicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.39
8
-
assay at
Methanothermobacter thermautotrophicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.5.1.4
NAD+
-
Methanothermobacter thermautotrophicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.39
Triton X-100
0.5%, stimulates
Methanothermobacter thermautotrophicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.39
expression in Escherichia coli
Methanothermobacter thermautotrophicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.5.1.4
NAD+
-
Methanothermobacter thermautotrophicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.B14
phosphate
8–20 mM phosphate buffer inhibits
Methanothermobacter thermautotrophicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.39
membrane
-
Methanothermobacter thermautotrophicus
16020
-
2.7.8.39
membrane
-
Pyrococcus furiosus
16020
-
3.1.3.B14
membrane
-
Methanothermobacter thermautotrophicus
16020
-
5.5.1.4
membrane
-
Methanothermobacter thermautotrophicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.39
Mg2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
2.7.8.39
Mn2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.39
20764
-
x * 20764, calculated from sequence
Methanothermobacter thermautotrophicus
2.7.8.39
20900
-
x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea
1-archaetidyl-1D-myo-inositol + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.5.1.4
partially purified by ammonium sulfate fractionation (50-70%)
Methanothermobacter thermautotrophicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.39
0.02
-
pH 8.0, 60°C, crude enzyme
Methanothermobacter thermautotrophicus
5.5.1.4
0.0003
-
crude enzyme, in 50mM Bicine buffer (pH 8.5), at 60°C
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
2.7.8.39
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
-
704559
Methanothermobacter thermautotrophicus
1-archaetidyl-1D-myo-inositol + phosphate
-
-
-
?
3.1.3.B14
1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea
704559
Methanothermobacter thermautotrophicus
1-archaetidyl-1D-myo-inositol + phosphate
-
-
-
?
5.5.1.4
D-glucose 6-phosphate
-
704559
Methanothermobacter thermautotrophicus
1L-myo-inositol 1-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.39
?
x * 20764, calculated from sequence; x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.39
60
-
assay at
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.39
8
-
assay at
Methanothermobacter thermautotrophicus