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Literature summary extracted from

  • Bonten, E.J.; Campos, Y.; Zaitsev, V.; Nourse, A.; Waddell, B.; Lewis, W.; Taylor, G.; dAzzo, A.
    Heterodimerization of the sialidase NEU1 with the chaperone protective protein/cathepsin A prevents its premature oligomerization (2009), J. Biol. Chem., 284, 28430-28441.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.18 protective protein/cathepsin A because of its association with protective protein/cathepsin A, which acts as a molecular chaperone, NEU1 is transported to the lysosomal compartment, catalytically activated, and stabilized Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed in Sf9 insect cells Homo sapiens
3.4.16.5 expressed in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.5 A251E the mutant shows increased activity compared to the wild type cathepsin A Homo sapiens
3.4.16.5 K355Q the mutant shows increased activity compared to the wild type cathepsin A Homo sapiens
3.4.16.5 L354D the mutant shows decreased activity compared to the wild type cathepsin A Homo sapiens
3.4.16.5 P451A the mutant shows increased activity compared to the wild type cathepsin A Homo sapiens
3.4.16.5 R20A the mutant shows decreased activity compared to the wild type cathepsin A Homo sapiens
3.4.16.5 W382A the mutant shows decreased activity compared to the wild type cathepsin A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.5 32000
-
 mature enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Homo sapiens
-
-
-
3.4.16.5 Homo sapiens P10619
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.5
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.16.5 fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Homo sapiens 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
3.4.16.5 lysosomal neuraminidase-1 + H2O lysosomal neuraminidase-1 gains full catalytic activity in the lysosome through its binding to PPCA Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 NEU1
-
 Homo sapiens
3.2.1.18 neuraminidase-1
-
 Homo sapiens
3.2.1.18 sialidase
-
 Homo sapiens
3.4.16.5 PpcA
-
 Homo sapiens
3.4.16.5 protective protein/cathepsin A
-
 Homo sapiens