BRENDA - Enzyme Database

Pantoate kinase and phosphopantothenate synthetase, two novel enzymes necessary for CoA biosynthesis in the archaea

Yokooji, Y.; Tomita, H.; Atomi, H.; Imanaka, T.; J. Biol. Chem. 284, 28137-28145 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.3.2.36
expression in Escherichia coli
Thermococcus kodakarensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
CoA
no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA
Thermococcus kodakarensis
2.7.1.169
additional information
no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate
Thermococcus kodakarensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.47
-
ATP
42°C
Thermococcus kodakarensis
2.7.1.169
1.2
-
Pantoate
42°C
Thermococcus kodakarensis
2.7.1.169
1.3
-
pantothenate
42°C
Thermococcus kodakarensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.169
32748
-
2 * 32748, calculated from sequence
Thermococcus kodakarensis
2.7.1.169
34000
-
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
6.3.2.36
29843
-
2 * 29843, calculated from sequence
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.169
ATP + pantoate
Thermococcus kodakarensis
-
ADP + 4-phosphopantoate
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
Thermococcus kodakarensis
the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism
AMP + diphosphate + 4'-phosphopantothenate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.169
Thermococcus kodakarensis
Q5JHF1
-
-
6.3.2.36
Thermococcus kodakarensis
Q5JIZ8
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.2.36
recombinant enzyme
Thermococcus kodakarensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.169
ATP + pantoate
-
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
ATP + pantoate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
ATP + pantothenate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
-
704547
Thermococcus kodakarensis
AMP + diphosphate + 4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism
704547
Thermococcus kodakarensis
AMP + diphosphate + 4'-phosphopantothenate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.169
dimer
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
2.7.1.169
dimer
2 * 32748, calculated from sequence
Thermococcus kodakarensis
6.3.2.36
dimer
2 * 29843, calculated from sequence
Thermococcus kodakarensis
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.169
PoK
-
Thermococcus kodakarensis
2.7.1.169
TK2141 protein
-
Thermococcus kodakarensis
6.3.2.36
phosphopantothenate synthetase
-
Thermococcus kodakarensis
6.3.2.36
Pps
-
Thermococcus kodakarensis
6.3.2.36
TK1686 protein
-
Thermococcus kodakarensis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
42
-
assay
Thermococcus kodakarensis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.21
-
pantothenate
42°C
Thermococcus kodakarensis
2.7.1.169
1.48
-
ATP
42°C
Thermococcus kodakarensis
2.7.1.169
1.56
-
Pantoate
42°C
Thermococcus kodakarensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.36
8
-
assay at
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.36
expression in Escherichia coli
Thermococcus kodakarensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
CoA
no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA
Thermococcus kodakarensis
2.7.1.169
additional information
no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate
Thermococcus kodakarensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.47
-
ATP
42°C
Thermococcus kodakarensis
2.7.1.169
1.2
-
Pantoate
42°C
Thermococcus kodakarensis
2.7.1.169
1.3
-
pantothenate
42°C
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.169
32748
-
2 * 32748, calculated from sequence
Thermococcus kodakarensis
2.7.1.169
34000
-
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
6.3.2.36
29843
-
2 * 29843, calculated from sequence
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.169
ATP + pantoate
Thermococcus kodakarensis
-
ADP + 4-phosphopantoate
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
Thermococcus kodakarensis
the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism
AMP + diphosphate + 4'-phosphopantothenate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.36
recombinant enzyme
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.169
ATP + pantoate
-
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
ATP + pantoate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
ATP + pantothenate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
-
704547
Thermococcus kodakarensis
AMP + diphosphate + 4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + 4-phosphopantoate + beta-alanine
the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism
704547
Thermococcus kodakarensis
AMP + diphosphate + 4'-phosphopantothenate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.169
dimer
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
2.7.1.169
dimer
2 * 32748, calculated from sequence
Thermococcus kodakarensis
6.3.2.36
dimer
2 * 29843, calculated from sequence
Thermococcus kodakarensis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
42
-
assay
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.21
-
pantothenate
42°C
Thermococcus kodakarensis
2.7.1.169
1.48
-
ATP
42°C
Thermococcus kodakarensis
2.7.1.169
1.56
-
Pantoate
42°C
Thermococcus kodakarensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.36
8
-
assay at
Thermococcus kodakarensis
General Information
EC Number
General Information
Commentary
Organism
2.7.1.169
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA
Thermococcus kodakarensis
6.3.2.36
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain can not grow at all in the absence of CoA
Thermococcus kodakarensis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.169
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA
Thermococcus kodakarensis
6.3.2.36
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain can not grow at all in the absence of CoA
Thermococcus kodakarensis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.17
-
pantothenate
42°C
Thermococcus kodakarensis
2.7.1.169
1.3
-
Pantoate
42°C
Thermococcus kodakarensis
2.7.1.169
3.1
-
ATP
42°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.17
-
pantothenate
42°C
Thermococcus kodakarensis
2.7.1.169
1.3
-
Pantoate
42°C
Thermococcus kodakarensis
2.7.1.169
3.1
-
ATP
42°C
Thermococcus kodakarensis