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Literature summary extracted from

  • OLeary, B.; Rao, S.K.; Kim, J.; Plaxton, W.C.
    Bacterial-type phosphoenolpyruvate carboxylase (PEPC) functions as a catalytic and regulatory subunit of the novel class-2 PEPC complex of vascular plants (2009), J. Biol. Chem., 284, 24797-24805.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.31 expressed in Escherichia coli BL21(DE3) cells Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 D-fructose 6-phosphate 99% residual activity at 2 mM Ricinus communis
4.1.1.31 D-glucose 1-phosphate 95% residual activity at 2 mM Ricinus communis
4.1.1.31 D-glucose 6-phosphate 93% residual activity at 2 mM Ricinus communis
4.1.1.31 glycerol 3-phosphate 98% residual activity at 2 mM Ricinus communis
4.1.1.31 L-aspartate 98% residual activity at 2 mM Ricinus communis
4.1.1.31 L-malate 84% residual activity at 2 mM Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.84
-
 phosphoenolpyruvate at pH 7.3 Ricinus communis
4.1.1.31 0.87
-
 phosphoenolpyruvate at pH 8.0 Ricinus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ absolutely dependent on, PPC4 exhibits a 4fold higher specific activity with saturating (10mM) Mg2+ relative to Mn2+ Ricinus communis
4.1.1.31 Mn2+ may replace Mg2+ Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 118000
-
 PPC4, gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.31 additional information Ricinus communis recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Ricinus communis A6YM33 PPC4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31 Ni-NTA column chromatography, Superose-6 gel filtration, and Superdex 200 gel filtration Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 seed
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 0.3
-
 clarified extract Ricinus communis
4.1.1.31 25.2
-
 after 152fold purification Ricinus communis

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 -20°C, 50% (v/v) glycerol, the final PPC4 preparation proves difficult to store as it slowly loses activity and is completely inactivated when rapidly thawed after freezing in liquid N2 Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 additional information recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs Ricinus communis ?
-
 ?
4.1.1.31 phosphoenolpyruvate + CO2
-
 Ricinus communis phosphate + oxaloacetate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 bacterial-type PEPC
-
 Ricinus communis
4.1.1.31 BTPC
-
 Ricinus communis
4.1.1.31 PEPC
-
 Ricinus communis
4.1.1.31 Phosphoenolpyruvate carboxylase
-
 Ricinus communis
4.1.1.31 PPC
-
 Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8 8.5
-
 Ricinus communis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.31 11
-
 at pH 7.0 Ricinus communis L-malate
4.1.1.31 33
-
 at pH 7.0 Ricinus communis L-aspartate