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Literature summary extracted from

  • Christensen, Q.H.; Cronan, J.E.
    The Thermoplasma acidophilum LplA-LplB complex defines a new class of bipartite lipoate-protein ligases (2009), J. Biol. Chem., 284, 21317-21326.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.20 expressed in Escherichia coli Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.20 11200
-
 1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration Thermoplasma acidophilum
6.3.1.20 30800
-
 1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration Thermoplasma acidophilum
6.3.1.20 41100
-
 LplA-LplB complex, gel filtration Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.20 additional information Thermoplasma acidophilum LplA has no detectable ligase activity in vitro in the absence of LplB ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.20 Thermoplasma acidophilum Q9HKT1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.20 Ni2+-NTA-agarose resin column chromatography and POROS HQ 20 column chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.20 ATP + D-lipoic acid
-
 Thermoplasma acidophilum diphosphate + D-lipoyl-AMP
-
 ?
6.3.1.20 additional information LplA has no detectable ligase activity in vitro in the absence of LplB Thermoplasma acidophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.20 heterodimer 1 * 30800 + 1 * 11200, LplA-LplB complex, gel filtration Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
6.3.1.20 lipoate ligase
-
 Thermoplasma acidophilum
6.3.1.20 lipoyl ligase
-
 Thermoplasma acidophilum
6.3.1.20 LPLA
-
 Thermoplasma acidophilum
6.3.1.20 LplA-LplB complex
-
 Thermoplasma acidophilum
6.3.1.20 LplB
-
 Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.20 ATP
-
 Thermoplasma acidophilum